PUBLICATION
Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System
- Authors
- Aksoy, Y.A., Yang, B., Chen, W., Hung, T., Kuchel, R.P., Zammit, N.W., Grey, S.T., Goldys, E.M., Deng, W.
- ID
- ZDB-PUB-201120-91
- Date
- 2020
- Source
- ACS applied materials & interfaces 12(47): 52433-52444 (Journal)
- Registered Authors
- Aksoy, Yagiz, Grey, Shane
- Keywords
- CRISPR-Cas9, light triggering, liposomes, protein release, temporal control
- MeSH Terms
-
- Animals
- CRISPR-Cas Systems/genetics*
- Embryo, Nonmammalian/metabolism
- Gene Editing/methods*
- Gene Expression/drug effects
- Genes, Reporter
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- HEK293 Cells
- Humans
- Light
- Liposomes/chemistry*
- Singlet Oxygen/metabolism
- Tumor Necrosis Factor alpha-Induced Protein 3/genetics
- Tumor Necrosis Factor alpha-Induced Protein 3/metabolism
- Tumor Necrosis Factor-alpha/pharmacology
- Zebrafish/growth & development
- Zebrafish/metabolism
- PubMed
- 33174413 Full text @ ACS Appl. Mater. Interfaces
Citation
Aksoy, Y.A., Yang, B., Chen, W., Hung, T., Kuchel, R.P., Zammit, N.W., Grey, S.T., Goldys, E.M., Deng, W. (2020) Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System. ACS applied materials & interfaces. 12(47):52433-52444.
Abstract
The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFα-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping