PUBLICATION

Morphogenesis is transcriptionally coupled to neurogenesis during peripheral olfactory organ development

Authors
Aguillon, R., Madelaine, R., Aguirrebengoa, M., Guturu, H., Link, S., Dufourcq, P., Lecaudey, V., Bejerano, G., Blader, P., Batut, J.
ID
ZDB-PUB-201120-23
Date
2020
Source
Development (Cambridge, England)   147(24): (Journal)
Registered Authors
Batut, Julie, Blader, Patrick, Dufourcq, Pascale, Lecaudey, Virginie, Madelaine, Romain
Keywords
Chemokine, Morphogenesis, Neurogenesis, Neurogenin1, Olfactory placode, Zebrafish
MeSH Terms
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/genetics*
  • E-Box Elements/genetics
  • Embryo, Nonmammalian
  • Embryonic Development/genetics
  • Gene Expression Regulation, Developmental/genetics
  • Morphogenesis/genetics*
  • Mutation/genetics
  • Nerve Tissue Proteins/genetics*
  • Neurogenesis/genetics*
  • Neurons/metabolism
  • Olfactory Mucosa/growth & development*
  • Receptors, CXCR4/genetics*
  • Transcription Initiation Site
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish Proteins/genetics*
PubMed
33144399 Full text @ Development
Abstract
Sense organs acquire their distinctive shapes concomitantly with the differentiation of sensory cells and neurons necessary for their function. While our understanding of the mechanisms controlling morphogenesis and neurogenesis in these structures has grown, how these processes are coordinated remains largely unexplored. Neurogenesis in the zebrafish olfactory epithelium requires the bHLH proneural transcription factor Neurogenin1 (Neurog1). To address whether Neurog1 also controls morphogenesis, we analysed the migratory behaviour of early olfactory neural progenitors in neurog1 mutant embryos. Our results indicate that the oriented movements of these progenitors are disrupted in this context. Morphogenesis is similarly affected by mutations in the chemokine receptor gene, cxcr4b, suggesting it is a potential Neurog1 target gene. We find that Neurog1 directly regulates cxcr4b through an E-boxes cluster located just upstream of the cxcr4b transcription start site. Our results suggest that proneural transcription factors, such as Neurog1, directly couple distinct aspects of nervous system development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping