PUBLICATION
Natural iridoids from Patrinia heterophylla showing anti-inflammatory activities in vitro and in vivo
- Authors
- Wu, P., Song, Z., Li, Y., Wang, H., Zhang, H., Bao, J., Li, Y., Cui, J., Jin, D.Q., Wang, A., Liang, B., Lee, D., Xu, J., Guo, Y.
- ID
- ZDB-PUB-201120-15
- Date
- 2020
- Source
- Bioorganic chemistry 104: 104331 (Journal)
- Registered Authors
- Cui, Jianlin, Li, Yuhao
- Keywords
- Anti-inflammatory potency, Iridoids, NO inhibition, Patrinia heterophylla, Zebrafish model
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents, Non-Steroidal/chemistry
- Anti-Inflammatory Agents, Non-Steroidal/isolation & purification
- Anti-Inflammatory Agents, Non-Steroidal/pharmacology*
- Biological Products/chemistry
- Biological Products/isolation & purification
- Biological Products/pharmacology*
- Cell Survival/drug effects
- Dose-Response Relationship, Drug
- Inflammation/drug therapy*
- Inflammation/metabolism
- Inflammation/pathology
- Iridoids/chemistry
- Iridoids/isolation & purification
- Iridoids/pharmacology*
- Mice
- Molecular Docking Simulation
- Molecular Structure
- Nitric Oxide/analysis
- Nitric Oxide/antagonists & inhibitors
- Nitric Oxide/biosynthesis
- Patrinia/chemistry*
- Reactive Oxygen Species/analysis
- Reactive Oxygen Species/antagonists & inhibitors
- Reactive Oxygen Species/metabolism
- Structure-Activity Relationship
- Zebrafish
- PubMed
- 33142407 Full text @ Bioorg. Chem.
Citation
Wu, P., Song, Z., Li, Y., Wang, H., Zhang, H., Bao, J., Li, Y., Cui, J., Jin, D.Q., Wang, A., Liang, B., Lee, D., Xu, J., Guo, Y. (2020) Natural iridoids from Patrinia heterophylla showing anti-inflammatory activities in vitro and in vivo. Bioorganic chemistry. 104:104331.
Abstract
Inflammation, especially chronic inflammation, has been found to be closely related to the pathology of many diseases and the discovery of bioactive natural products to inhibit NO production is one of strategies to treat inflammation. In our continuous search for bioactive natural substances as potential anti-inflammatory agents, five new compounds (1-5) were extracted and purified from Patrinia heterophylla. The NMR and MS data analysis, along with electronic circular dichroism (ECD) calculations, led to the identification of these isolates, which were new iridoids. Using cell and zebrafish models, the in vitro and in vivo anti-inflammatory effects were conducted to evaluate the potency of anti-inflammation of these compounds. The preliminary mechanism was explored using molecular docking and Western blotting experiments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping