PUBLICATION
Carcinogen-induced model of pro-angiogenesis in zebrafish embryo-larvae
- Authors
- Pawar, N.V., Singh, P.D., Prabhu, P.S., Rana, J.R.
- ID
- ZDB-PUB-201120-102
- Date
- 2020
- Source
- Environmental toxicology and chemistry 40(2): 447-453 (Journal)
- Registered Authors
- Pawar, Nilambari
- Keywords
- angiogenesis, benzo-a-pyrene, carcinogen, lindane, tumor, zebrafish
- MeSH Terms
-
- Angiogenesis Inhibitors/pharmacology
- Animals
- Carcinogens/toxicity
- Embryo, Nonmammalian*
- Larva
- Zebrafish*
- PubMed
- 33179787 Full text @ Environ. Toxicol. Chem.
Citation
Pawar, N.V., Singh, P.D., Prabhu, P.S., Rana, J.R. (2020) Carcinogen-induced model of pro-angiogenesis in zebrafish embryo-larvae. Environmental toxicology and chemistry. 40(2):447-453.
Abstract
Tumor angiogenesis is the main target in cancer drug development. Discovery of anti-angiogenic agents targeting different mechanisms of action is the major area of research to control tumor growth and metastasis. Zebrafish (in embryo-larvae stage) acts as an essential pre-clinical efficacy-toxicity model for anti-angiogenic drug discovery. Herein, we aimed to develop a carcinogen-induced model of pro-angiogenesis in zebrafish embryo-larvae using carcinogens lindane and benzo-a-pyrene. Zebrafish were randomly selected for mating. Post-spawning, healthy embryos were staged, dispensed in reverse osmosis water in a 12-well plate, and incubated at 28.5°C, wherein 24 hours post fertilization, the embryos were exposed to sub-lethal concentrations of the carcinogens. Three days post exposure, the embryos were stained with alkaline phosphatase, and the angiogenic basket was imaged using bright-field microscope. Number of sub-intestinal vessels, their length from somite to the basket, and other pro-angiogenic parameters were measured and analyzed. The effective concentration (EC30) for increase in sub-intestinal vessels for benzo-a-pyrene and lindane was 2.69 and 2.24 µM, respectively, thus proving their pro-angiogenic potency. Carcinogen-induced model of pro-angiogenesis in zebrafish embryo-larvae can be used as an effective high-throughput screening tool for assessing pro-angiogenic potential of carcinogenic compounds and to screen anti-angiogenic drugs for better therapeutic intervention. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping