ZFIN ID: ZDB-PUB-201029-6
Tissue-Specific In Vivo Biotin Chromatin Immunoprecipitation with Sequencing in Zebrafish and Chicken
Lukoseviciute, M., Ling, I.T.C., Senanayake, U., Candido-Ferreira, I., Taylor, G., Williams, R.M., Sauka-Spengler, T.
Date: 2020
Source: STAR protocols   1: 100066 (Journal)
Registered Authors: Sauka-Spengler, Tatjana
Keywords: none
Microarrays: GEO:GSE106676
MeSH Terms: none
PubMed: 33111104 Full text @ STAR Protoc
ABSTRACT
Chromatin immunoprecipitation with sequencing (ChIP-seq) has been instrumental in understanding transcription factor (TF) binding during gene regulation. ChIP-seq requires specific antibodies against desired TFs, which are not available for numerous species. Here, we describe a tissue-specific biotin ChIP-seq protocol for zebrafish and chicken embryos which utilizes AVI tagging of TFs, permitting their biotinylation by a co-expressed nuclear biotin ligase. Subsequently, biotinylated factors can be precipitated with streptavidin beads, enabling the user to construct TF genome-wide binding landscapes like conventional ChIP-seq methods. For complete details on the use and execution of this protocol, please see Lukoseviciute et al. (2018) and Ling and Sauka-Spengler (2019).
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