PUBLICATION
Dose-dependent Changes After Proton and Photon Irradiation in a Zebrafish Model
- Authors
- Brunner, S., TŐkÉs, T., SzabÓ, E.R., Polanek, R., SzabÓ, I.Z., Reisz, Z., GubÁn, B.K., SzijÁrtÓ, A.L., Brand, M., Hans, S., Karsch, L., Lessmann, E., Pawelke, J., SchÜrer, M., Beyreuther, E., HideghÉty, K.
- ID
- ZDB-PUB-201029-3
- Date
- 2020
- Source
- Anticancer research 40: 6123-6135 (Journal)
- Registered Authors
- Brand, Michael, Hans, Stefan
- Keywords
- DNA-DSB, Proton, histological changes, morphological malformations, photon, zebrafish embryo model
- MeSH Terms
-
- Animals
- Brain/radiation effects
- DNA Damage
- Disease Models, Animal
- Dose-Response Relationship, Radiation
- Embryo, Nonmammalian/radiation effects
- Eye/pathology
- Eye/radiation effects
- Kinetics
- Organ Size/radiation effects
- Photons*
- Protons*
- Relative Biological Effectiveness
- Yolk Sac/pathology
- Yolk Sac/radiation effects
- Zebrafish/embryology
- Zebrafish/physiology*
- PubMed
- 33109550 Full text @ Anticancer Res.
Citation
Brunner, S., TŐkÉs, T., SzabÓ, E.R., Polanek, R., SzabÓ, I.Z., Reisz, Z., GubÁn, B.K., SzijÁrtÓ, A.L., Brand, M., Hans, S., Karsch, L., Lessmann, E., Pawelke, J., SchÜrer, M., Beyreuther, E., HideghÉty, K. (2020) Dose-dependent Changes After Proton and Photon Irradiation in a Zebrafish Model. Anticancer research. 40:6123-6135.
Abstract
Background/aim The importance of hadron therapy in the cancer management is growing. We aimed to refine the biological effect detection using a vertebrate model.
Materials and methods Embryos at 24 and 72 h postfertilization were irradiated at the entrance plateau and the mid spread-out Bragg peak of a 150 MeV proton beam and with reference photons. Radiation-induced DNA double-strand breaks (DSB) and histopathological changes of the eye, muscles and brain were evaluated; deterioration of specific organs (eye, yolk sac, body) was measured.
Results More and longer-lasting DSBs occurred in eye and muscle cells due to proton versus photon beams, albeit in different numbers. Edema, necrosis and tissue disorganization, (especially in the eye) were observed. Dose-dependent morphological deteriorations were detected at ≥10 Gy dose levels, with relative biological effectiveness between 0.99±0.07 (length) and 1.12±0.19 (eye).
Conclusion Quantitative assessment of radiation induced changes in zebrafish embryos proved to be beneficial for the radiobiological characterization of proton beams.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping