PUBLICATION
Short-term exposure to the simple polyphenolic compound gallic acid induces neuronal hyperactivity in zebrafish larvae
- Authors
- Annona, G., Tarallo, A., Nittoli, V., Varricchio, E., Sordino, P., D'Aniello, S., Paolucci, M.
- ID
- ZDB-PUB-201025-6
- Date
- 2020
- Source
- The European journal of neuroscience 53(5): 1367-1377 (Other)
- Registered Authors
- D'Aniello, Salvatore, Sordino, Paolo
- Keywords
- c-fos, GABA, Phenolic compounds, glutamate, neurotransmitters
- MeSH Terms
-
- Animals
- Gallic Acid*/toxicity
- Larva
- Neurons
- Prosencephalon
- Zebrafish*
- PubMed
- 33098676 Full text @ Eur. J. Neurosci.
Citation
Annona, G., Tarallo, A., Nittoli, V., Varricchio, E., Sordino, P., D'Aniello, S., Paolucci, M. (2020) Short-term exposure to the simple polyphenolic compound gallic acid induces neuronal hyperactivity in zebrafish larvae. The European journal of neuroscience. 53(5):1367-1377.
Abstract
A growing body of evidence suggests that the biological effects of polyphenols are not restricted to antioxidant activity, but they exert a wide range of modulatory effects on metabolic pathways, cellular signaling and gene expression. In this study we tested the minimum safe concentration of gallic acid in 72 hpf zebrafish larvae in order to evaluate the effects on the central nervous system and the behavioral response. We showed that a short-exposure (30 minutes) induces the depletion of the two main excitatory and inhibitory neurotransmitters, Glu and GABA respectively, in the larval nervous system. The acute impairment of GABAergic-glutaminergic balance was paralleled by an increase of the fosab neuronal activity marker in specific brain areas, such as the forebrain, olfactory bulbs, pallial area, ventral midbrain, tegmentum, and the medulla oblongata ventral area. The neuronal excitation was mirrored by the increased cumulative motor response. The inhibition of the olfactory epithelium with brief cadmium exposition suggests a direct involvement of olfaction in the larvae response to gallic acid. Our results demonstrate that a brief exposure to gallic acid induces motoneuronal hyperexcitability in zebrafish. The behavioral response was probably elicited through the activation of an odorous, or chemical stimulus. The specificity of the activated neuronal territories suggests the involvement of additional signaling pathways. Although the underlying molecular mechanisms remain to be elucidated, our data support the hypothesis that GA acts as an excitatory molecule, capable of inducing a specific nerve response. These results offer a new vision on potential effects of gallic acid.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping