PUBLICATION
Skin swabbing is a refined technique to collect DNA from model fish species
- Authors
- Tilley, C.A., Carreño Gutierrez, H., Sebire, M., Obasaju, O., Reichmann, F., Katsiadaki, I., Barber, I., Norton, W.H.J.
- ID
- ZDB-PUB-201025-4
- Date
- 2020
- Source
- Scientific Reports 10: 18212 (Journal)
- Registered Authors
- Norton, Will, Tilley, Ceinwen
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA/genetics
- DNA/isolation & purification*
- Hydrocortisone/metabolism
- Models, Biological*
- Smegmamorpha/genetics*
- Zebrafish/genetics*
- PubMed
- 33097784 Full text @ Sci. Rep.
Citation
Tilley, C.A., Carreño Gutierrez, H., Sebire, M., Obasaju, O., Reichmann, F., Katsiadaki, I., Barber, I., Norton, W.H.J. (2020) Skin swabbing is a refined technique to collect DNA from model fish species. Scientific Reports. 10:18212.
Abstract
Model fish species such as sticklebacks and zebrafish are frequently used in studies that require DNA to be collected from live animals. This is typically achieved by fin clipping, a procedure that is simple and reliable to perform but that can harm fish. An alternative procedure to sample DNA involves swabbing the skin to collect mucus and epithelial cells. Although swabbing appears to be less invasive than fin clipping, it still requires fish to be netted, held in air and handled-procedures that can cause stress. In this study we combine behavioural and physiological analyses to investigate changes in gene expression, behaviour and welfare after fin clipping and swabbing. Swabbing led to a smaller change in cortisol release and behaviour on the first day of analysis compared to fin clipping. It also led to less variability in data suggesting that fewer animals need to be measured after using this technique. However, swabbing triggered some longer term changes in zebrafish behaviour suggesting a delayed response to sample collection. Skin swabbing does not require the use of anaesthetics and triggers fewer changes in behaviour and physiology than fin clipping. It is therefore a more refined technique for DNA collection with the potential to improve fish health and welfare.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping