PUBLICATION
Cystic fibrosis transmembrane conductance regulator (CFTR) regulates embryonic organizer formation during zebrafish early embryogenesis
- Authors
- Liu, Y., Lin, Z., Sun, H.
- ID
- ZDB-PUB-201020-34
- Date
- 2020
- Source
- The International journal of developmental biology 64: 409-413 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cystic Fibrosis Transmembrane Conductance Regulator/genetics
- Cystic Fibrosis Transmembrane Conductance Regulator/metabolism*
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism*
- Gene Expression Regulation, Developmental
- Mutation
- Proteome/genetics
- Proteome/metabolism*
- Proteomics/methods*
- Wnt Signaling Pathway/genetics
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- beta Catenin/genetics
- beta Catenin/metabolism
- PubMed
- 33063835 Full text @ Int. J. Dev. Biol.
Citation
Liu, Y., Lin, Z., Sun, H. (2020) Cystic fibrosis transmembrane conductance regulator (CFTR) regulates embryonic organizer formation during zebrafish early embryogenesis. The International journal of developmental biology. 64:409-413.
Abstract
Cystic fibrosis (CF) is associated with the manifestation of a number of medical conditions throughout the body. This prompted us to investigate the etiology of CF from the viewpoint of the embryonic organizer, which is responsible for steering the movement of surrounding cells into specific organs and tissues. In our previous work, we found that a cftr mutant had decreased nuclear β-catenin levels in the early embryo at 5 hours post-fertilization (hpf), when the organizer forms. It is known that nuclear β-catenin signaling is essential for the induction of the dorsal organizer. Therefore, we explored the role of cftr in the formation of the embryonic organizer in this work. Indeed, the expression of organizer and germ layer markers was significantly affected in cftr mutant embryos dependent on Wnt/β-catenin signaling. Furthermore, quantitative proteome analysis revealed that the cftr mutant induced significant alteration in the expression of proteins related to many critical biological processes, cellular components, molecular functions, and signaling pathways, except for the Wnt/β-catenin pathway. These findings demonstrate the function of cftr in embryonic organizer formation and provide an explanation for why many abnormalities occur in the bodies of CF patients.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping