PUBLICATION

Interplay of MPP5a with Rab11 synergistically builds epithelial apical polarity and zonula adherens

Authors

Hao, Y., Zhou, Y., Yu, Y., Zheng, M., Weng, K., Kou, Z., Liang, J., Zhang, Q., Tang, X., Xu, P., Link, B.A., Yao, K., Zou, J.

ID

ZDB-PUB-201020-24

Date

2020

Source

Development (Cambridge, England) 147(22): (Journal)

Registered Authors

Keywords

Adherens junctions remodeling, Apical polarity, MPP5a, Rab11, Zonula adherens

MeSH Terms

Adherens Junctions/genetics
Adherens Junctions/metabolism*
Animals
Cell Polarity/physiology*
Epithelial Cells
Guanylate Cyclase/genetics
Guanylate Cyclase/metabolism*
Cell Movement/physiology*
Zebrafish/embryology*
Zebrafish/genetics
rab GTP-Binding Proteins/genetics
rab GTP-Binding Proteins/metabolism*
Protein Transport/physiology
Golgi Apparatus/genetics
Golgi Apparatus/metabolism
Cadherins/genetics
Cadherins/metabolism
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*

(all 19)

PubMed

33060129 Full text @ Development

Abstract

Adherens junctions remodeling regulated by apical polarity proteins constitutes a major driving force for tissue morphogenesis, though the precise mechanism remains inconclusive. Here we reported that Crumbs complex component MPP5a interacts with small GTPase Rab11 in Golgi to synergistically transport cadherin and Crumbs components to the apical domain, thus establishing the apical epithelial polarity and adherens junctions. In contrast, Par complex recruited by MPP5a is incapable to interact with Rab11 but may assemble cytoskeleton to facilitate the cadherin exocytosis. In accordance, dysfunction of MPP5a induced an invasive migration of epithelial cells. This adherens junctions remodeling pattern is frequently observed in zebrafish lens epithelial cells and neuroepithelial cells. The data identified an unrecognized MPP5a/Rab11 complex and described its essential role in guiding the apical polarization and zonula adherens formation in epithelial cells.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
pals1a^{zju203/zju203}	standard conditions	Prim-25	lens adherens junction absent, abnormal	Fig. 2
pals1a^{zju203/zju203}	standard conditions	Prim-25	lens epithelial cell decreased amount, abnormal	Fig. 2
pals1a^{zju203/zju203}	standard conditions	Prim-25	lens epithelial cell mislocalised, abnormal	Fig. 2
pals1a^{zju203/zju203}	standard conditions	Prim-25	lens epithelium pals1a expression absent, abnormal	Fig. 2
pals1a^{zju203/zju203}	standard conditions	Prim-25	lens epithelium adherens junction mislocalised, abnormal	Fig. 2
pals1a^{zju203/zju203}	standard conditions	Prim-25	lens epithelium apical part of cell crb2a expression mislocalised, abnormal	Fig. 6
pals1a^{zju203/zju203}	standard conditions	Prim-25	lens epithelium apical part of cell prkci expression absent, abnormal	Fig. 6
pals1a^{zju203/zju203}	standard conditions	Prim-25	lens fiber cell increased amount, abnormal	Fig. 2
prkci^m567/m567	standard conditions	Prim-25	lens epithelium apical part of cell crb2a expression mislocalised, abnormal	Fig. 6
prkci^m567/m567	standard conditions	Prim-25	lens epithelium apical part of cell prkci expression absent, abnormal	Fig. 6

1 - 10 of 21

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
m289	Point Mutation	crb2a
m567	Point Mutation	prkci
zju203	Indel	pals1a
zju233	Indel	rab11a
zju234	Small Deletion	rab11ba

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
pals1a	CRISPR1-pals1a	CRISPR
rab11a	CRISPR1-rab11a	CRISPR

Fish

Fish
pals1a^{zju203/zju203}
prkci^m567/m567
rab11a^{zju233/zju233}
rab11ba^{zju234/zju234}
rab11ba^{zju234/zju234}; rab11a^{zju233/zju233}
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Hao et al., 2020 ZDB-PUB-201020-24 PMID:33060129

Interplay of MPP5a with Rab11 synergistically builds epithelial apical polarity and zonula adherens

Hao et al., 2020

ZDB-PUB-201020-24
PMID:33060129