PUBLICATION
Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration
- Authors
- Alhashem, Z., Portillo, M.A., Htun, M.R., Gauert, A., Montecinos, L.B., Härtel, S., Linker, C.
- ID
- ZDB-PUB-201002-84
- Date
- 2021
- Source
- Methods in molecular biology (Clifton, N.J.) 2179: 79-106 (Review)
- Registered Authors
- Linker, Claudia
- Keywords
- Cell dissociation, Cell migration, Cell tracking, Clonal analysis, Gal4/Kalt4, In vivo imaging, Photoconversion, Tamoxifen, UAS, Zebrafish
- MeSH Terms
-
- Animals
- Cell Movement/genetics*
- Embryonic Development/genetics
- Molecular Biology/methods*
- Neural Crest/ultrastructure*
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish Proteins/ultrastructure*
- PubMed
- 32939715 Full text @ Meth. Mol. Biol.
Citation
Alhashem, Z., Portillo, M.A., Htun, M.R., Gauert, A., Montecinos, L.B., Härtel, S., Linker, C. (2021) Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration. Methods in molecular biology (Clifton, N.J.). 2179:79-106.
Abstract
The study of cell migration has been greatly enhanced by the development of new model systems and analysis protocols to study this process in vivo. Zebrafish embryos have been a principal protagonist because they are easily accessible, genetically tractable, and optically transparent. Neural crest cells, on the other hand, are the ideal system to study cell migration. These cells migrate extensively, using different modalities of movement and sharing many traits with metastatic cancer cells. In this chapter, we present new tools and protocols that allow the study of NC development and migration in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping