PUBLICATION
Fast, easy and early (larval) identification of transparent mutant zebrafish using standard fluorescence microscopy
- Authors
- Wenz, R., Conibear, E., Bugeon, L., Dallman, M.
- ID
- ZDB-PUB-201002-71
- Date
- 2020
- Source
- F1000Research 9: 963 (Journal)
- Registered Authors
- Bugeon, Laurence, Dallman, Maggie
- Keywords
- Zebrafish, casper, iridophore, nac, screening, tra, trab6/b6nacw2/w2, translucent, transparent
- MeSH Terms
-
- Animals
- Larva/genetics
- Microscopy, Fluorescence
- Reference Standards
- Zebrafish*/genetics
- PubMed
- 32934809 Full text @ F1000Res
Citation
Wenz, R., Conibear, E., Bugeon, L., Dallman, M. (2020) Fast, easy and early (larval) identification of transparent mutant zebrafish using standard fluorescence microscopy. F1000Research. 9:963.
Abstract
The availability of transparent zebrafish mutants (either TraNac: tra b6/b6; nac w2/w2 or casper: roy a9/a9; nac w2/w2 ) for live imaging studies together with the ease of generating transgenic lines are two of the strengths of the zebrafish model organism. The fact that transparent casper ( roy a9/a9;nac w2/w2) and silver nacre ( nac w2/w2) mutants are indistinguishable by eye at early stages (1-5 days post-fertilization; dpf) means many fish must be raised and later culled if they are not transparent. To identify translucent mutants early and easily at the early larval stage (≤5 dpf) before they are classified as protected animals, we developed a simple screening method using standard fluorescence microscopy. We estimate that this procedure could annually save 60,000 animals worldwide.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping