PUBLICATION
Identification of novel fish sialidase genes responsible for KDN-cleaving activity
- Authors
- Shiozaki, K., Uezono, K., Hirai, G., Honda, A., Minoda, M., Wakata, R.
- ID
- ZDB-PUB-201002-184
- Date
- 2020
- Source
- Glycoconjugate journal 37(6): 745-753 (Journal)
- Registered Authors
- Shiozaki, Kazuhiro
- Keywords
- KDN, Neu3, Neu4, Sialic acid, Sialidase
- MeSH Terms
-
- Animals
- Characidae/genetics
- Cichlids/genetics
- Cloning, Molecular
- Humans
- Hydrolysis
- Neuraminidase/chemistry
- Neuraminidase/genetics*
- Sialic Acids/metabolism*
- Substrate Specificity/genetics
- Sugar Acids/chemistry
- Sugar Acids/metabolism*
- Zebrafish/genetics
- PubMed
- 32980954 Full text @ Glycoconj. J.
Citation
Shiozaki, K., Uezono, K., Hirai, G., Honda, A., Minoda, M., Wakata, R. (2020) Identification of novel fish sialidase genes responsible for KDN-cleaving activity. Glycoconjugate journal. 37(6):745-753.
Abstract
2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) is a minor component of sialic acids detected in vertebrates, such as human cancer cells, rat liver, and fish tissues. Although the enzyme activity of KDN-cleaving sialidase (KDN-sialidase) has been detected in rainbow trout, the gene responsible for its expression has not been identified in vertebrates. We evaluated sialidases in human and various fish for their KDN-cleaving activity using an artificial substrate, methylumbelliferyl-KDN (MU-KDN). Four of the human sialidases tested (NEU1, NEU2, NEU3, and NEU4) did not hydrolyze MU-KDN. Although most fish Neu1s showed negligible KDN-sialidase activity, two Neu1b sialidases from Oreochromis niloticus and Astyanax mexicanus, a paralog of Neu1, exhibited a potent KDN-sialidase activity. Further, O. niloticus and Oryzias latipes Neu3a exhibited a drastically high KDN-sialidase activity, while Danio rerio Neu3.1 showed moderate activities and other Neu3 proteins exhibited little activity. All the Neu4 sialidases tested in fish cleaved KDN and Neu5Ac from MU-KDN and MU-Neu5Ac, respectively, with equivalent potential. To our knowledge, this is the first report to identify KDN-sialidase genes in vertebrates and we believe that KDN-sialidase activity could be conserved among fish Neu4s.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping