PUBLICATION
Succinyl chitosan gold nanocomposite: Preparation, characterization, in vitro and in vivo anticandidal activity
- Authors
- Dananjaya, S.H.S., Edirisinghe, S.L., Thao, N.T.T., Kumar, R.S., Wijerathna, H.M.S.M., Mudiyanselage, A.Y., De Zoysa, M., Choi, D.
- ID
- ZDB-PUB-201002-152
- Date
- 2020
- Source
- International journal of biological macromolecules 165(Pt A): 63-70 (Journal)
- Registered Authors
- Keywords
- Antifungal activity, Candida albicans, Gold nanocomposite, N-succinyl chitosan, Zebrafish
- MeSH Terms
-
- Animals
- Antifungal Agents/chemistry*
- Antifungal Agents/pharmacology
- Candida albicans/drug effects*
- Candida albicans/pathogenicity
- Chitosan/chemistry*
- Chitosan/pharmacology
- Drug Carriers/chemistry
- Drug Carriers/pharmacology
- Gold/chemistry
- HEK293 Cells
- Humans
- Nanocomposites/chemistry*
- Silver/chemistry
- X-Ray Diffraction
- PubMed
- 32971172 Full text @ Int. J. Biol. Macromol.
Citation
Dananjaya, S.H.S., Edirisinghe, S.L., Thao, N.T.T., Kumar, R.S., Wijerathna, H.M.S.M., Mudiyanselage, A.Y., De Zoysa, M., Choi, D. (2020) Succinyl chitosan gold nanocomposite: Preparation, characterization, in vitro and in vivo anticandidal activity. International journal of biological macromolecules. 165(Pt A):63-70.
Abstract
Herein, we have successfully synthesized a novel N-Succinyl chitosan/gold nanocomposite (N-SuC/Au NC) using N-SuC and gold(III) chloride, and investigated the biocompatibility and antifungal activity. The synthesized N-SuC/Au NC was characterized by UV-visible spectroscopy, X-ray diffraction, field emission scanning electron microscope, and inductively coupled plasma atomic emission spectroscopy. The N-SuC/Au NC exhibited a strong inhibition effect towards pathogenic Candida albicans. Morphological analysis revealed the destruction of C. albicans cell membrane due to N-SuC/Au NC treatment. The in vitro and in vivo toxicity of N-SuC/Au NC was analyzed with HEK293T mammalian cells and zebrafish larvae, respectively. The synthesized N-SuC/Au NC demonstrated no cytotoxicity towards HEK293T cells up to 1200 μg/mL concentration. The survival rate of the zebrafish larvae at 120 hpf, was found as 100% up to 1200 μg/mL of N-SuC/Au NC exposure. The in vivo studies further confirmed the inhibitory effects of N-SuC/Au NC on the formation of C. albicans hyphae in zebrafish muscle tissue.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping