ZFIN ID: ZDB-PUB-200829-12
A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish
Sun, F., Shoffner, A.R., Poss, K.D.
Date: 2021
Source: Methods in molecular biology (Clifton, N.J.)   2158: 71-80 (Chapter)
Registered Authors: Poss, Kenneth D., Shoffner, Adam, Sun, Fei
Keywords: Ablation, Cardiomyocyte, Heart, Regeneration, Tamoxifen, Zebrafish
MeSH Terms:
  • Animals
  • Catheter Ablation/adverse effects*
  • Cell Proliferation
  • Disease Models, Animal*
  • Heart Injuries/etiology
  • Heart Injuries/pathology*
  • Heart Injuries/rehabilitation
  • Myocytes, Cardiac/cytology*
  • Myocytes, Cardiac/physiology
  • Regeneration*
  • Ventricular Remodeling*
  • Zebrafish
  • Zebrafish Proteins/genetics*
PubMed: 32857367 Full text @ Meth. Mol. Biol.
Adult zebrafish possess an elevated cardiac regenerative capacity as compared with adult mammals. In the past two decades, zebrafish have provided a key model system for studying the cellular and molecular mechanisms of innate heart regeneration. The ease of genetic manipulation in zebrafish has enabled the establishment of a genetic ablation injury model in which over 60% of cardiomyocytes can be depleted, eliciting signs of heart failure. After this severe injury, adult zebrafish efficiently regenerate lost cardiomyocytes and reverse heart failure. In this chapter, we describe the methods for inducing genetic cardiomyocyte ablation in adult zebrafish, assessing cardiomyocyte proliferation, and histologically analyzing regeneration after injury.