ZFIN ID: ZDB-PUB-200829-11
Cardiac Resection Injury in Zebrafish
Sheng, D.Z., Zheng, D., Kikuchi, K.
Date: 2021
Source: Methods in molecular biology (Clifton, N.J.)   2158: 63-69 (Chapter)
Registered Authors: Kikuchi, Kazu, Sheng, Delicia
Keywords: Cardiomyocytes, Heart failure, Heart regeneration, Injury, Myocardial infarction, Zebrafish
MeSH Terms:
  • Animals
  • Cardiac Surgical Procedures/adverse effects*
  • Cell Proliferation
  • Disease Models, Animal*
  • Heart/physiology*
  • Heart Injuries/etiology
  • Heart Injuries/pathology*
  • Heart Injuries/surgery
  • Ventricular Remodeling*
  • Zebrafish
PubMed: 32857366 Full text @ Meth. Mol. Biol.
The zebrafish (Danio rerio) possesses a spectacular capacity for cardiac regeneration. Zebrafish have been used in cardiac regeneration research for nearly two decades, contributing to the identification of signals and cellular mechanisms as potential targets for human heart repair. Investigations into cardiac regeneration in zebrafish have been facilitated by multiple methods of inducing cardiac tissue damage. Among the established methods, cardiac resection injury is a relatively simple, yet robust approach traditionally used to induce cardiac tissue damage in a reproducible manner. Here, we describe a detailed protocol to perform a cardiac resection injury in adult zebrafish and discuss potential complications for researchers who are new to this technique.