PUBLICATION

Protocol for Dissection and Dissociation of Zebrafish Telencephalon for Single-Cell Sequencing

Authors
Cosacak, M.I., Bhattarai, P., Kizil, C.
ID
ZDB-PUB-200825-11
Date
2020
Source
STAR protocols   1: 100042 (Other)
Registered Authors
Bhattarai, Prabesh, Cosacak, Mehmet Ilyas, Kizil, Caghan
Keywords
none
MeSH Terms
  • Animals
  • Dissection/methods*
  • Humans
  • Single-Cell Analysis/methods*
  • Telencephalon/cytology*
  • Zebrafish/surgery*
PubMed
32835293 Full text @ STAR Protoc
Abstract
Single-cell sequencing (sc-Seq) is a powerful tool to investigate the molecular signatures of cell types in a complex mixture of cells. A critical step in sc-Seq is preparing a single-cell suspension with a high number of viable cells. Here, we show how to dissect zebrafish telencephalon and how to dissociate it into a single-cell suspension. This is followed by flow cytometry-based sorting to enrich for neural progenitor stem cells. Our technique typically yields 70,000 live cells from one zebrafish telencephalon. For complete details on the use and execution of this protocol, please refer to Cosacak et al. (2019).
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes