Generation of foxn1/Casper Mutant Zebrafish for Allograft and Xenograft of Normal and Malignant Cells
- Lv, P., Ma, D., Gao, S., Zhang, Y., Bae, Y.K., Liang, G., Gao, S., Choi, J.H., Kim, C.H., Wang, L., Liu, F.
- Stem Cell Reports 15(3): 749-760 (Journal)
- Registered Authors
- Bae, Young Ki, Choi, Jung-Hwa, Gao, Shuai, Kim, Cheol-Hee, Liu, Feng, Lv, Peng, Ma, Dongyuan, Zhang, Yifan
- foxn1/Casper mutant, hematopoietic stem cells, nonconditioned cell transplantation, xenograft, zebrafish
- MeSH Terms
- Base Sequence
- Fetal Stem Cells/cytology
- Forkhead Transcription Factors/genetics*
- Forkhead Transcription Factors/metabolism
- Hematopoietic Stem Cell Transplantation
- Hematopoietic Stem Cells/cytology
- Treatment Outcome
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- 32822590 Full text @ Stem Cell Reports
Lv, P., Ma, D., Gao, S., Zhang, Y., Bae, Y.K., Liang, G., Gao, S., Choi, J.H., Kim, C.H., Wang, L., Liu, F. (2020) Generation of foxn1/Casper Mutant Zebrafish for Allograft and Xenograft of Normal and Malignant Cells. Stem Cell Reports. 15(3):749-760.
Cell transplantation into immunodeficient recipients is a widely used approach to study stem cell and cancer biology; however, studying cell states post transplantation in vivo is inconvenient in mammals. Here, we generated a foxn1/Casper mutant zebrafish that is transparent and exhibits T cell deficiency. By employing the line for hematopoietic stem cell (HSC) transplantation (HSCT), we could achieve nonconditioned transplantation. Meanwhile, we found that fetal HSCs from 3 days post fertilization zebrafish embryos produce a better transplant outcome in foxn1/Casper mutants, compared with adult HSCs. In addition to HSCT, the foxn1/Casper mutant is feasible for allografts of myelodysplastic syndrome-like and muscle cells, as well as xenografts of medaka muscle cells. In summary, foxn1/Casper mutants permit the nonconditioned engraftment of multiple cell types and visualized characterization of transplanted cells in vivo.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes