PUBLICATION
Fluorous soluble cyanine dyes for visualizing perfluorocarbons in living systems
- Authors
- Lim, I., Vian, A., van de Wouw, H., Day, R.A., Gomez, C., Liu, Y., Rheingold, A.L., Campas, O., Sletten, E.M.
- ID
- ZDB-PUB-200819-8
- Date
- 2020
- Source
- Journal of the American Chemical Society 142(37): 16072-16081 (Journal)
- Registered Authors
- Gomez, Carlos, Vian, Antoine
- Keywords
- none
- MeSH Terms
-
- Animals
- Carbocyanines/chemical synthesis
- Carbocyanines/chemistry*
- Fluorescent Dyes/chemical synthesis
- Fluorescent Dyes/chemistry*
- Fluorocarbons/analysis*
- Microscopy, Fluorescence
- Molecular Structure
- Optical Imaging
- Solubility
- Zebrafish
- PubMed
- 32808518 Full text @ J. Am. Chem. Soc.
Citation
Lim, I., Vian, A., van de Wouw, H., Day, R.A., Gomez, C., Liu, Y., Rheingold, A.L., Campas, O., Sletten, E.M. (2020) Fluorous soluble cyanine dyes for visualizing perfluorocarbons in living systems. Journal of the American Chemical Society. 142(37):16072-16081.
Abstract
The bioorthogonal nature of perfluorocarbons provides a unique platform for introducing dynamic nano- and microdroplets into cells and organisms. To monitor the localization and deformation of the droplets, fluorous soluble fluorophores that are compatible with standard fluorescent protein markers and applicable to cells, tissues, and small organisms are necessary. Here, we introduce fluorous cyanine dyes that represent the most red-shifted fluorous soluble fluorophores to date. We study the effect of covalently appended fluorous tags on the cyanine scaffold and evaluate the changes in photophysical properties imparted by the fluorous phase. Ultimately, we showcase the utility of the fluorous soluble pentamethine cyanine dye for tracking the localization of perfluorocarbon nanoemulsions in macrophage cells and for measurements of mechanical forces in multicellular spheroids and zebrafish embryonic tissues. These studies demonstrate that the red-shifted cyanine dyes offer spectral flexibility in multiplexed imaging experiments and enhanced precision in force measurements.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping