PUBLICATION

Assessment of Vascular Patterning in the Zebrafish

Authors
Stratman, A.N., Weinstein, B.M.
ID
ZDB-PUB-200807-6
Date
2021
Source
Methods in molecular biology (Clifton, N.J.)   2206: 205-222 (Chapter)
Registered Authors
Stratman, Amber, Weinstein, Brant M.
Keywords
Blood vessel, Endothelium, Lymphatic vessel, Microangiography, Morpholino, Vascular biology, Vasculature, Zebrafish
MeSH Terms
  • Angiography/methods*
  • Animals
  • Blood Vessels/metabolism
  • Blood Vessels/physiology*
  • Morpholinos/metabolism
  • Neovascularization, Physiologic/physiology
  • Phenotype
  • Zebrafish/metabolism
  • Zebrafish/physiology*
  • Zebrafish Proteins/metabolism
PubMed
32754820 Full text @ Meth. Mol. Biol.
Abstract
The zebrafish has emerged as a valuable and important model organism for studying vascular development and vascular biology. Here, we discuss some of the approaches used to study vessels in fish, including loss-of-function tools such as morpholinos and genetic mutants, along with methods and considerations for assessing vascular phenotypes. We also provide detailed protocols for methods used for vital imaging of the zebrafish vasculature, including microangiography and long-term time-lapse imaging. The methods we describe, and the considerations we suggest using for assessing phenotypes observed using these methods, will help ensure reliable, valid conclusions when assessing vascular phenotypes following genetic or experimental manipulation of zebrafish.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes