PUBLICATION
Isolation, structural elucidation, and immunoregulation properties of an arabinofuranan from the rinds of Garcinia mangostana
- Authors
- Zhang, S., Li, Z., Wang, X., An, L., Bao, J., Zhang, J., Cui, J., Li, Y., Jin, D.Q., Tuerhong, M., Abudukeremu, M., Ohizumi, Y., Xu, J., Guo, Y.
- ID
- ZDB-PUB-200805-3
- Date
- 2020
- Source
- Carbohydrate Polymers 246: 116567 (Journal)
- Registered Authors
- Cui, Jianlin
- Keywords
- 2', 7'-dichlorofluorescein diacetate (PubChem CID: 104913), 3-amino, 4-aminomethyl-2′, 7′-difluorescein diacetate (PubChem CID: 2762646), Conformational analysis, Dimethyl sulfoxide (PubChem CID: 679), GC–MS, Garcinia mangostana L., Immunoregulation, Macrophages, Methyl iodide (PubChem CID: 6328), NMR, Penicillin (PubChem CID: 2349), Polysaccharide, Sodium borohydride (PubChem CID: 4311764), Sodium chloride (PubChem CID: 5234), Streptomycin (PubChem CID: 19649), Tricaine (PubChem CID: 261501), Trifluoroacetic acid (PubChem CID: 6422), Zebrafish embryo
- MeSH Terms
-
- Animals
- Cell Survival/drug effects
- Cytokines/metabolism
- Garcinia mangostana/chemistry*
- Immunologic Factors/chemistry*
- Immunologic Factors/isolation & purification
- Immunologic Factors/pharmacology*
- Macrophages/drug effects
- Macrophages/metabolism
- Mice
- Molecular Weight
- Monosaccharides
- Nitric Oxide/metabolism
- Plant Extracts/chemistry*
- Plant Extracts/isolation & purification
- Plant Extracts/pharmacology*
- Polysaccharides/chemistry*
- Polysaccharides/isolation & purification
- Polysaccharides/pharmacology*
- RAW 264.7 Cells
- Reactive Oxygen Species/metabolism
- Signal Transduction/drug effects*
- Zebrafish/metabolism
- PubMed
- 32747240 Full text @ Carbohydr. Polym.
Citation
Zhang, S., Li, Z., Wang, X., An, L., Bao, J., Zhang, J., Cui, J., Li, Y., Jin, D.Q., Tuerhong, M., Abudukeremu, M., Ohizumi, Y., Xu, J., Guo, Y. (2020) Isolation, structural elucidation, and immunoregulation properties of an arabinofuranan from the rinds of Garcinia mangostana. Carbohydrate Polymers. 246:116567.
Abstract
In our search for bioactive polysaccharides as immunomodulatory agents, an arabinofuranan (GMP90-1) was purified and characterized from the rinds of Garcinia mangostana L. GMP90-1 (absolute molecular weight: 5.30 × 103 g/mol) was found to be composed of arabinose, galactose, and rhamnose. The backbone of GMP90-1 was determined as (1→5)-linked α-l-Araf, (1→2,3,5)-linked α-l-Araf, (1→3,5)-linked α-l-Araf, (1→6)-linked β-d-Galp, and (1→2)-linked α-l-Rhap. Conformational analysis revealed GMP90-1 to exist as a rigid rod structure in sodium chloride solution. To explore its potential as immunomodulatory agents, an in vitro cell screening was performed and GMP90-1 was found to significantly enhance the phagocytic uptake of neutral red and improve the secreted level of nitric oxide (NO), interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) of macrophages. Furthermore, the cellular immunomodulatory activities were confirmed by the in vivo zebrafish experiment, which suggested that GMP90-1 with immunomodulatory effects could be considered as a potential immunomodulatory for immune diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping