ZFIN ID: ZDB-PUB-200801-3
Dopaminergic neurons regenerate following chemogenetic ablation in the olfactory bulb of adult Zebrafish (Danio rerio)
Godoy, R., Hua, K., Kalyn, M., Cusson, V.M., Anisman, H., Ekker, M.
Date: 2020
Source: Scientific Reports   10: 12825 (Journal)
Registered Authors: Ekker, Marc
Keywords: none
MeSH Terms:
  • Ablation Techniques/adverse effects
  • Ablation Techniques/methods
  • Animals
  • Animals, Genetically Modified
  • Dopaminergic Neurons/drug effects*
  • Dopaminergic Neurons/physiology*
  • Metronidazole/adverse effects*
  • Nerve Regeneration/physiology*
  • Olfactory Bulb/cytology*
  • Olfactory Bulb/physiology*
  • Prodrugs/adverse effects*
  • Smell/drug effects*
  • Smell/physiology*
  • Time Factors
  • Zebrafish/physiology*
PubMed: 32733000 Full text @ Sci. Rep.
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ABSTRACT
Adult zebrafish have the ability to regenerate cells of the central nervous system. However, few neuronal regeneration studies in adult zebrafish addressed their ability to regenerate specific types of neurons following cell specific ablation. We show here that treatment of transgenic Tg(dat:CFP-NTR) adult zebrafish with the prodrug metronidazole (Mtz) according to our administration regimen predominantly ablates dopamine (DA) neurons within the olfactory bulb (OB) of adult fish. Loss of DA neurons was accompanied by an impaired olfaction phenotype, as early as 1-week post-treatment, in which fish were unable to sense the presence of the repulsive stimulus cadaverine. The olfactory impairment was reversed within 45 days and coincided with the recovery of DA neuron counts in the OB. A multi-label pulse-chase analysis with BrdU and EdU over the first seventeen days-post Mtz exposure showed that newly formed DA neurons were recruited within the first nine days following exposure and led to functional and morphological recovery of the OB.
ADDITIONAL INFORMATION