PUBLICATION
A heteropolysaccharide purified from leaves of Ilex latifolia displaying immunomodulatory activity in vitro and in vivo
- Authors
- Shi, Z., An, L., Zhang, S., Li, Z., Li, Y., Cui, J., Zhang, J., Jin, D.Q., Tuerhong, M., Abudukeremu, M., Xu, J., Guo, Y.
- ID
- ZDB-PUB-200729-2
- Date
- 2020
- Source
- Carbohydrate Polymers 245: 116469 (Journal)
- Registered Authors
- Cui, Jianlin
- Keywords
- Chloroform (PubChem CID: 6212), D-Galactose (PubChem CID: 6036), D-Glucose (PubChem CID: 5793), D-Mannose (PubChem CID: 18950), Dimethyl sulfoxide (PubChem CID: 679), Ilex latifolia, Immunoregulatory activity, L-Arabinose (PubChem CID: 439195), Methyl iodide (PubChem CID: 6328), Neutral red (PubChem CID: 11105), Polysaccharide, Thiazolyl Blue Tetrazolium Bromide (PubChem CID: 64965), Trifluoroacetic acid (PubChem CID: 6422), Zebrafish
- MeSH Terms
-
- Animals
- Cell Survival/drug effects
- Cytokines/metabolism
- Ilex/chemistry*
- Immunologic Factors/chemistry
- Immunologic Factors/isolation & purification*
- Immunologic Factors/pharmacology*
- Macrophages/drug effects*
- Macrophages/immunology
- Mice
- Molecular Weight
- Monosaccharides
- Nitric Oxide/metabolism
- Phagocytosis/drug effects
- Plant Leaves/chemistry*
- Polysaccharides/chemistry
- Polysaccharides/isolation & purification*
- Polysaccharides/pharmacology*
- RAW 264.7 Cells
- Signal Transduction/drug effects
- Zebrafish/embryology
- Zebrafish/metabolism
- PubMed
- 32718604 Full text @ Carbohydr. Polym.
Citation
Shi, Z., An, L., Zhang, S., Li, Z., Li, Y., Cui, J., Zhang, J., Jin, D.Q., Tuerhong, M., Abudukeremu, M., Xu, J., Guo, Y. (2020) A heteropolysaccharide purified from leaves of Ilex latifolia displaying immunomodulatory activity in vitro and in vivo. Carbohydrate Polymers. 245:116469.
Abstract
A novel polysaccharide (ILP50-2) was extracted, isolated and purified from the leaves of Ilex latifolia Thunb. Its structure was characterized as a repeating unit consisting of α-L-Araf-(1→, →3)-α-L-Araf-(1→, →5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, β-D-Galp-(1→, →4)-β-D-Galp-(1→, →4)-β-D-Glcp-(1→, →6)-α-D-Manp-(1→, and →3,6)-α-D-Galp-(1→. The absolute molecular weight of ILP50-2 was 1.49 × 105 g/mol, which adapted a compact coil conformation in 0.1 M NaCl solution with Rz of 25.4 nm. Furthermore, ILP50-2 exhibited immunoregulatory activity, mainly through enhancing the phagocytosis ability of macrophages and prompting the release of nitric oxide (NO) and cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). Simultaneously, ILP50-2 was found to significantly increase the release of ROS and NO in zebrafish embryos, showing immunoregulatory effects in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping