PUBLICATION
In vivo tracking cystine/glutamate antiporter-mediated cysteine/cystine pool under ferroptosis
- Authors
- Li, Z., Li, Y., Yang, Y., Gong, Z., Zhu, H., Qian, Y.
- ID
- ZDB-PUB-200719-3
- Date
- 2020
- Source
- Analytica chimica acta 1125: 66-75 (Journal)
- Registered Authors
- Gong, Zhiyuan, Li, Zhen
- Keywords
- Cysteine/glutamate transporter (system X(C)(−)), Ferroptosis, Fluorescent imaging strategy, Intracellular cysteine/cystine pool, Zebrafish
- MeSH Terms
-
- Amino Acid Transport Systems/metabolism*
- Animals
- Antiporters/metabolism*
- Benzylidene Compounds/chemical synthesis
- Benzylidene Compounds/chemistry
- Cell Line, Tumor
- Cysteine/analysis*
- Ferroptosis/physiology*
- Fluorescent Dyes/chemical synthesis
- Fluorescent Dyes/chemistry*
- Humans
- Microscopy, Fluorescence
- Nitriles/chemical synthesis
- Nitriles/chemistry
- Optical Imaging
- Zebrafish
- PubMed
- 32674782 Full text @ Anal. Chim. Acta
Citation
Li, Z., Li, Y., Yang, Y., Gong, Z., Zhu, H., Qian, Y. (2020) In vivo tracking cystine/glutamate antiporter-mediated cysteine/cystine pool under ferroptosis. Analytica chimica acta. 1125:66-75.
Abstract
Cysteine/cystine redox couple plays a vital role in maintaining the cellular redox state of living bio-systems. However, the dynamic changes of the intracellular cysteine/cystine pool mediated by cysteine/glutamate transporter (System XC-) in various cellular processes have remained incompletely understood. Here we established a series of strategies around fluorescent probe CP2 for effectively monitoring the intracellular cysteine level in living cells and in vivo zebrafish model, therefore for tracking dynamic changes of system XC--mediated cysteine/cystine pool under ferroptosis. Living cell imaging and in vivo visualization both revealed that intracellular cysteine levels could be significantly reduced upon blocking system XC- activity and inducing ferroptotic cell death. This kind of blocking was potent against cisplatin-resistant A549R cancer cells, which might bring new potential for treating drug-resistant carcinoma through regulating system XC- and processes of ferroptosis. With further improvements in monitoring tools, understanding of system XC--mediated cystine/cysteine pool and ferroptosis could certainly offer crucial information in more physiological or pathological processes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping