PUBLICATION
Tuberculosis causes highly conserved metabolic changes in human patients, mycobacteria-infected mice and zebrafish larvae
- Authors
- Ding, Y., Raterink, R.J., Marín-Juez, R., Veneman, W.J., Egbers, K., van den Eeden, S., Haks, M.C., Joosten, S.A., Ottenhoff, T.H.M., Harms, A.C., Alia, A., Hankemeier, T., Spaink, H.P.
- ID
- ZDB-PUB-200717-5
- Date
- 2020
- Source
- Scientific Reports 10: 11635 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amines/analysis*
- Amines/chemistry
- Animals
- Chromatography, Liquid
- Disease Models, Animal
- Glucose/metabolism*
- Humans
- Larva/metabolism
- Larva/microbiology
- Least-Squares Analysis
- Magnetic Resonance Spectroscopy
- Mass Spectrometry
- Mice
- Mice, Inbred C57BL
- Mycobacterium marinum
- Mycobacterium tuberculosis
- Tuberculosis/metabolism*
- Zebrafish/metabolism*
- Zebrafish/microbiology
- PubMed
- 32669636 Full text @ Sci. Rep.
Citation
Ding, Y., Raterink, R.J., Marín-Juez, R., Veneman, W.J., Egbers, K., van den Eeden, S., Haks, M.C., Joosten, S.A., Ottenhoff, T.H.M., Harms, A.C., Alia, A., Hankemeier, T., Spaink, H.P. (2020) Tuberculosis causes highly conserved metabolic changes in human patients, mycobacteria-infected mice and zebrafish larvae. Scientific Reports. 10:11635.
Abstract
Tuberculosis is a highly infectious and potentially fatal disease accompanied by wasting symptoms, which cause severe metabolic changes in infected people. In this study we have compared the effect of mycobacteria infection on the level of metabolites in blood of humans and mice and whole zebrafish larvae using one highly standardized mass spectrometry pipeline, ensuring technical comparability of the results. Quantification of a range of circulating small amines showed that the levels of the majority of these compounds were significantly decreased in all three groups of infected organisms. Ten of these metabolites were common between the three different organisms comprising: methionine, asparagine, cysteine, threonine, serine, tryptophan, leucine, citrulline, ethanolamine and phenylalanine. The metabolomic changes of zebrafish larvae after infection were confirmed by nuclear magnetic resonance spectroscopy. Our study identified common biomarkers for tuberculosis disease in humans, mice and zebrafish, showing across species conservation of metabolic reprogramming processes as a result of disease. Apparently, the mechanisms underlying these processes are independent of environmental, developmental and vertebrate evolutionary factors. The zebrafish larval model is highly suited to further investigate the mechanism of metabolic reprogramming and the connection with wasting syndrome due to infection by mycobacteria.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping