ZFIN ID: ZDB-PUB-200711-14
Tendon Cell Regeneration Is Mediated by Attachment Site-Resident Progenitors and BMP Signaling
Niu, X., Subramanian, A., Hwang, T.H., Schilling, T.F., Galloway, J.L.
Date: 2020
Source: Current biology : CB   30(17): 3277-3292.e5 (Journal)
Registered Authors: Galloway, Jenna, Schilling, Tom, Subramanian, Arul
Keywords: BMP, ablation, nkx2.5, progenitor cells, regeneration, scleraxis, sox10, tendon, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/growth & development*
  • Animals, Genetically Modified/metabolism
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism*
  • Cell Differentiation
  • Musculoskeletal System/metabolism*
  • Regeneration*
  • Stem Cells/cytology*
  • Stem Cells/metabolism
  • Tendons/cytology*
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
PubMed: 32649909 Full text @ Curr. Biol.
The musculoskeletal system is a striking example of how cell identity and position is coordinated across multiple tissues to ensure function. However, it is unclear upon tissue loss, such as complete loss of cells of a central musculoskeletal connecting tendon, whether neighboring tissues harbor progenitors capable of mediating regeneration. Here, using a zebrafish model, we genetically ablate all embryonic tendon cells and find complete regeneration of tendon structure and pattern. We identify two regenerative progenitor populations, sox10+ perichondrial cells surrounding cartilage and nkx2.5+ cells surrounding muscle. Surprisingly, laser ablation of sox10+ cells, but not nkx2.5+ cells, increases tendon progenitor number in the perichondrium, suggesting a mechanism to regulate attachment location. We find BMP signaling is active in regenerating progenitor cells and is necessary and sufficient for generating new scxa+ cells. Our work shows that muscle and cartilage connective tissues harbor progenitor cells capable of fully regenerating tendons, and this process is regulated by BMP signaling.