PUBLICATION
Hypoxia imaging in living cells, tissues and zebrafish with a nitroreductase-specific fluorescent probe
- Authors
- Fan, L., Zan, Q., Lin, B., Wang, X., Gong, X., Zhao, Z., Shuang, S., Dong, C., Wong, M.S.
- ID
- ZDB-PUB-200710-10
- Date
- 2020
- Source
- The Analyst 145(16): 5657-5663 (Journal)
- Registered Authors
- Zhao, Zhonghua
- Keywords
- none
- MeSH Terms
-
- Animals
- Fluorescent Dyes*/toxicity
- Humans
- Hypoxia
- Microscopy, Fluorescence
- Nitroreductases
- Zebrafish*
- PubMed
- 32638730 Full text @ Analyst
Citation
Fan, L., Zan, Q., Lin, B., Wang, X., Gong, X., Zhao, Z., Shuang, S., Dong, C., Wong, M.S. (2020) Hypoxia imaging in living cells, tissues and zebrafish with a nitroreductase-specific fluorescent probe. The Analyst. 145(16):5657-5663.
Abstract
Hypoxia in solid tumors is directly linked to the elevated levels of endogenous nitroreductase (NTR). We present a novel fluorescent probe, namely NTNO, for nitroreductase-specific detection based on the NTR-catalyzed reduction of the nitro unit to an amine functionality, and demonstrated its application for hypoxia imaging. NTNO was designed by incorporating a nitro unit as the NTR response site into a benzothiazole derivative. Upon reacting with NTR in the presence of reduced nicotinamide adenine dinucleotide (NADH), the fluorescence of the probe was strongly and sensitively turned on, with a good linearity in the NTR concentration range of 0.5-8.0 μg mL-1 and a detection limit of 48 ng mL-1. Most notably, NTNO has been successfully used for imaging hypoxia levels in living cells, tumor tissues and zebrafish, making it of great potential to monitor NTR in biological systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping