PUBLICATION
Potential Molecular Mechanisms and Drugs for Aconitine-Induced Cardiotoxicity in Zebrafish through RNA Sequencing and Bioinformatics Analysis
- Authors
- Wang, M., Shi, Y., Yao, L., Li, Q., Wang, Y., Fu, D.
- ID
- ZDB-PUB-200701-8
- Date
- 2020
- Source
- Medical science monitor : international medical journal of experimental and clinical research 26: e924092 (Journal)
- Registered Authors
- Wang, Youhua
- Keywords
- none
- MeSH Terms
-
- Aconitine/pharmacology
- Aconitine/toxicity*
- Animals
- Biomarkers, Tumor/genetics
- Cardiotoxicity/genetics*
- Computational Biology/methods
- Databases, Genetic
- Exome Sequencing/methods
- Gene Expression Profiling/methods
- Gene Ontology
- Gene Regulatory Networks/genetics
- Heart Rate/drug effects
- Molecular Docking Simulation
- Protein Interaction Mapping/methods
- Protein Interaction Maps/genetics
- Sequence Analysis, RNA/methods
- Transcriptome/genetics
- Zebrafish/genetics
- PubMed
- 32598336 Full text @ Med. Sci. Monit.
- CTD
- 32598336
Citation
Wang, M., Shi, Y., Yao, L., Li, Q., Wang, Y., Fu, D. (2020) Potential Molecular Mechanisms and Drugs for Aconitine-Induced Cardiotoxicity in Zebrafish through RNA Sequencing and Bioinformatics Analysis. Medical science monitor : international medical journal of experimental and clinical research. 26:e924092.
Abstract
BACKGROUND Accumulating evidence suggests that cardiotoxicity is one of the main manifestations of aconitine (AC) poisoning. However, the molecular mechanism of AC-induced cardiotoxicity remains unclear, there is little direct evidence for therapeutic targets and drugs of AC-induced cardiotoxicity. MATERIAL AND METHODS Zebrafish were exposed to AC to evaluate cardiotoxicity by calculating the heart rates and observing the changes of cardiac and vascular structure. RNA-seq (RNA sequencing) and bioinformatics analysis were used to obtain differentially expressed genes (DEGs). The anti-AC cardiotoxicity compound was identified via connectivity map (CMAP) analysis and molecular docking. RESULTS AC-induced cardiotoxicity in zebrafish predominantly included arrhythmias, extended sinus venous and bulbus arteriosus (SV-BA) distance, and larger pericardial edema aera. A total of 1380 DEGs were identified by RNA-seq and bioinformatics analysis. cyclin-dependent kinase-1 (CDK1) was screened as the hub gene and the most potential therapeutic target due to its significant downregulation in cardiotoxicity based on protein-protein interaction (PPI) and drug-gene interaction (DGIdb) network analysis. Cell cycle signal pathway was the most significant pathways identified in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Furthermore, the expression of CDK1 was validated in the Gene Expression Omnibus (GEO) database GSE71906, GSE65705, and GSE95140. Finally, heptaminol was identified as a novel anti-AC cardiotoxicity compound via CMAP analysis and molecular docking. CONCLUSIONS Totally, hub genes and key pathways identified in this study can aid in the understanding of the molecular changes in AC-induced cardiotoxicity. Meanwhile, we provide a systematic method to explore drug toxicity prevention and treatment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping