|ZFIN ID: ZDB-PUB-200613-11|
Midkine-a functions as a universal regulator of proliferation during epimorphic regeneration in adult zebrafish
Ang, N.B., Saera-Vila, A., Walsh, C., Hitchcock, P.F., Kahana, A., Thummel, R., Nagashima, M.
|Source:||PLoS One 15: e0232308 (Journal)|
|Registered Authors:||Hitchcock, Peter, Kahana, Alon, Nagashima, Mikiko, Thummel, Ryan|
|PubMed:||32530962 Full text @ PLoS One|
Ang, N.B., Saera-Vila, A., Walsh, C., Hitchcock, P.F., Kahana, A., Thummel, R., Nagashima, M. (2020) Midkine-a functions as a universal regulator of proliferation during epimorphic regeneration in adult zebrafish. PLoS One. 15:e0232308.
ABSTRACTZebrafish have the ability to regenerate damaged cells and tissues by activating quiescent stem and progenitor cells or reprogramming differentiated cells into regeneration-competent precursors. Proliferation among the cells that will functionally restore injured tissues is a fundamental biological process underlying regeneration. Midkine-a is a cytokine growth factor, whose expression is strongly induced by injury in a variety of tissues across a range of vertebrate classes. Using a zebrafish Midkine-a loss of function mutant, we evaluated regeneration of caudal fin, extraocular muscle and retinal neurons to investigate the function of Midkine-a during epimorphic regeneration. In wildtype zebrafish, injury among these tissues induces robust proliferation and rapid regeneration. In Midkine-a mutants, the initial proliferation in each of these tissues is significantly diminished or absent. Regeneration of the caudal fin and extraocular muscle is delayed; regeneration of the retina is nearly completely absent. These data demonstrate that Midkine-a is universally required in the signaling pathways that convert tissue injury into the initial burst of cell proliferation. Further, these data highlight differences in the molecular mechanisms that regulate epimorphic regeneration in zebrafish.