ZFIN ID: ZDB-PUB-200609-5
GFP expression pattern in pituitary and gonads under the control of nuclear progesterone receptor promoter in transgenic zebrafish
Huang, J., Zhang, T.T., Jiang, K., Hong, W.S., Chen, S.X.
Date: 2020
Source: Developmental dynamics : an official publication of the American Association of Anatomists   249(11): 1365-1376 (Journal)
Registered Authors: Chen, Shi-Xi
Keywords: gonad, nuclear progesterone receptor, pituitary, promoter, transgenic GFP expression, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified*/embryology
  • Animals, Genetically Modified*/genetics
  • Embryo, Nonmammalian/embryology*
  • Female
  • Gene Expression Regulation, Developmental*
  • Gonads/embryology*
  • Green Fluorescent Proteins*/biosynthesis
  • Green Fluorescent Proteins*/genetics
  • Male
  • Pituitary Gland/embryology*
  • Promoter Regions, Genetic*
  • Receptors, Progesterone/genetics*
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Zebrafish Proteins/genetics*
PubMed: 32506585 Full text @ Dev. Dyn.
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ABSTRACT
The nuclear progesterone receptor (Pgr) is a ligand-dependent transcription factor primarily responsible for mediating progesterone actions relevant for reproduction across vertebrates. Information on the cellular localization of Pgr expression in the reproductive system is required for developing a comprehensive approach to elucidate the role of Pgr in reproduction.
We generated transgenic zebrafish Tg(pgr:eGFP) that express enhanced green fluorescent protein (eGFP) driven by promoter sequence of pgr gene. The tissue distribution pattern of egfp mRNA is consistent with the pgr mRNA expression in Tg(pgr:eGFP). In the pituitary, GFP signals are found in the proximal pars distalis. In order to better discern the cellular localization of GFP signals in gonads, Tg(pgr:eGFP) line was crossed with Tg(gsdf:nfsB-mCherry) line, specifically expressing nitroreductase-mCherry fusion protein in granulosa and Sertoli cells in ovary and testis, respectively. Imaging of testis tissue showed that GFP expression was confined to Leydig cells. In the ovary, GFP expression co-localized with the mCherry signal in granulosa cells. Intriguingly, we also identified some non-granulosa cells close to where blood vessels branched, expressing stronger GFP signals than granulosa cells.
Analyzing Tg(pgr:eGFP) expression in zebrafish provided leads towards new routes to study the role of Pgr in reproduction. This article is protected by copyright. All rights reserved.
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