ZFIN ID: ZDB-PUB-200606-30
An NMR-based biosensor to measure stereo-specific methionine sulfoxide reductase (MSR) activities in vitro and in vivo
Sanchez-Lopez, C., Labadie, N., Lombardo, V., Biglione, F., Manta, B., Jacob, R., Gladyshev, V., Abdelilah-Seyfried, S., Selenko, P., Binolfi, A.
Date: 2020
Source: Chemistry (Weinheim an der Bergstrasse, Germany)   26(65): 14838-14843 (Journal)
Registered Authors: Abdelilah-Seyfried, Salim, Gladyshev, Vadim
Keywords: diastereoisomers, in-cell NMR, methionine oxidation, methionine sulfoxide reductase, zebrafish
MeSH Terms:
  • Biosensing Techniques*
  • Humans
  • Methionine
  • Methionine Sulfoxide Reductases/metabolism
  • Oxidation-Reduction
  • Substrate Specificity
PubMed: 32501570 Full text @ Chemistry
ABSTRACT
Oxidation of protein methionines to methionine-sulfoxides (MetOx) is associated with several age-related diseases. In healthy cells, MetOx is reduced to methionine by two families of conserved methionine sulfoxide reductase enzymes, MSRA and MSRB that specifically target the S - or R -diastereoisomers of methionine-sulfoxides, respectively. To directly interrogate MSRA and MSRB functions in cellular settings, we developed an NMR-based biosensor that we call CarMetOx to simultaneously measure both enzyme activities in single reaction setups. We demonstrate the suitability of our strategy to delineate MSR functions in complex biological environments, including cell lysates and live zebrafish embryos. Thereby, we establish differences in substrate specificities between prokaryotic and eukaryotic MSRs and introduce CarMetOx as a highly sensitive tool for studying therapeutic targets of oxidative stress-related human diseases and redox regulated signaling pathways.
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