PUBLICATION
Real-time 3D movement correction for two-photon imaging in behaving animals
- Authors
- Griffiths, V.A., Valera, A.M., Lau, J.Y., Roš, H., Younts, T.J., Marin, B., Baragli, C., Coyle, D., Evans, G.J., Konstantinou, G., Koimtzis, T., Nadella, K.M.N.S., Punde, S.A., Kirkby, P.A., Bianco, I.H., Silver, R.A.
- ID
- ZDB-PUB-200603-15
- Date
- 2020
- Source
- Nature Methods 17(7): 741-748 (Journal)
- Registered Authors
- Bianco, Isaac, Lau, Joanna
- Keywords
- none
- MeSH Terms
-
- Animals
- Female
- Imaging, Three-Dimensional/methods*
- Male
- Mice
- Mice, Inbred C57BL
- Microscopy, Fluorescence, Multiphoton/methods*
- Movement
- Zebrafish
- PubMed
- 32483335 Full text @ Nat. Methods
Citation
Griffiths, V.A., Valera, A.M., Lau, J.Y., Roš, H., Younts, T.J., Marin, B., Baragli, C., Coyle, D., Evans, G.J., Konstantinou, G., Koimtzis, T., Nadella, K.M.N.S., Punde, S.A., Kirkby, P.A., Bianco, I.H., Silver, R.A. (2020) Real-time 3D movement correction for two-photon imaging in behaving animals. Nature Methods. 17(7):741-748.
Abstract
Two-photon microscopy is widely used to investigate brain function across multiple spatial scales. However, measurements of neural activity are compromised by brain movement in behaving animals. Brain motion-induced artifacts are typically corrected using post hoc processing of two-dimensional images, but this approach is slow and does not correct for axial movements. Moreover, the deleterious effects of brain movement on high-speed imaging of small regions of interest and photostimulation cannot be corrected post hoc. To address this problem, we combined random-access three-dimensional (3D) laser scanning using an acousto-optic lens and rapid closed-loop field programmable gate array processing to track 3D brain movement and correct motion artifacts in real time at up to 1 kHz. Our recordings from synapses, dendrites and large neuronal populations in behaving mice and zebrafish demonstrate real-time movement-corrected 3D two-photon imaging with submicrometer precision.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping