PUBLICATION

Unifying Developmental Programs for Embryonic and Post-Embryonic Neurogenesis in the Zebrafish Retina

Authors
Xu, B., Tang, X., Jin, M., Zhang, H., Du, L., Yu, S., He, J.
ID
ZDB-PUB-200530-7
Date
2020
Source
Development (Cambridge, England)   147(12): (Journal)
Registered Authors
Du, Lei, He, Jie, Jin, Mengmeng, Tang, Xia, Xu, Baijie, Yu, Shuguang, Zhang, Hui
Keywords
Developmental state, Retinal progenitor cells, Retinal stem cells, Single-cell RNA sequencing, Zebrafish
Datasets
GEO:GSE122680
MeSH Terms
  • Gene Expression Regulation, Developmental
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Embryonic Development
  • Neurogenesis/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Embryo, Nonmammalian/metabolism*
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Sequence Analysis, RNA
  • Retina/cytology
  • Retina/growth & development
  • Retina/metabolism*
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • Single-Cell Analysis
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Fatty Acid-Binding Proteins/deficiency
  • Fatty Acid-Binding Proteins/genetics
  • Fatty Acid-Binding Proteins/metabolism
  • Animals
(all 28)
PubMed
32467236 Full text @ Development
Abstract
The zebrafish retina grows for a lifetime. Whether embryonic and post-embryonic retinogenesis conform to the same developmental program is an outstanding question that remains under debate. Using single-cell RNA sequencing of ∼20,000 cells of the developing zebrafish retina at four different stages, we identified seven distinct developmental states. Each state explicitly expresses a gene set. Disruption of individual state-specific marker genes results in various defects ranging from small eyes to the loss of distinct retinal cell types. Using a similar approach, we further characterized the developmental states of post-embryonic retinal stem cells (RSCs) and their progeny in the ciliary marginal zone. Expression pattern analysis of state-specific marker genes showed that the developmental states of post-embryonic RSCs largely recapitulated those of their embryonic counterparts, except for some differences in rod photoreceptor genesis. Thus, our findings reveal the unifying developmental program used by the embryonic and post-embryonic retinogenesis in zebrafish.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cu2TgTransgenic Insertion
    cu3314TgTransgenic Insertion
      ion9hTgTransgenic Insertion
        jh1TgTransgenic Insertion
          q20TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            bhlhe40CRISPR1-bhlhe40CRISPR
            bhlhe40CRISPR2-bhlhe40CRISPR
            bhlhe40CRISPR3-bhlhe40CRISPR
            bhlhe40CRISPR4-bhlhe40CRISPR
            fabp4aCRISPR5-fabp4aCRISPR
            fabp4aCRISPR3-fabp4aCRISPR
            fabp4aCRISPR4-fabp4aCRISPR
            mab21l2CRISPR1-mab21l2CRISPR
            mab21l2CRISPR2-mab21l2CRISPR
            mab21l2CRISPR3-mab21l2CRISPR
            1 - 10 of 15
            Show
            Fish
            No data available
            Antibodies
            Name Type Antigen Genes Isotypes Host Organism
            Ab5-casp3polyclonal
              		Rabbit
              Ab5-rhomonoclonalMouse
              1 - 2 of 2
              Show
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              CFPEFGCFP
              EGFPEFGEGFP
              GFPEFGGFP
              mCherryEFGmCherry
              NTREFGNTR
              RFPEFGRFP
              1 - 6 of 6
              Show
              Mapping
              No data available
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              Citation
              Xu, B., Tang, X., Jin, M., Zhang, H., Du, L., Yu, S., He, J. (2020) Unifying Developmental Programs for Embryonic and Post-Embryonic Neurogenesis in the Zebrafish Retina. Development (Cambridge, England). 147(12):.