PUBLICATION
Application of phage therapy: Synergistic effect of phage EcSw (ΦEcSw) and antibiotic combination towards antibiotic-resistant Escherichia coli
- Authors
- Easwaran, M., De Zoysa, M., Shin, H.J.
- ID
- ZDB-PUB-200527-6
- Date
- 2020
- Source
- Transboundary and Emerging diseases 67(6): 2809-2817 (Journal)
- Registered Authors
- Keywords
- Escherichia coli, antibiotics, phage-based therapy, ΦEcSw
- MeSH Terms
-
- Ampicillin/therapeutic use
- Animals
- Anti-Bacterial Agents/therapeutic use*
- Bacteriophages/physiology
- Chloramphenicol/therapeutic use
- Combined Modality Therapy
- Drug Resistance, Multiple, Bacterial/drug effects*
- Escherichia coli Infections/drug therapy*
- Escherichia coli Infections/veterinary
- Escherichia coli O157/drug effects*
- Kanamycin/therapeutic use
- Mice
- Mice, Inbred BALB C
- Microbial Sensitivity Tests
- Microbial Viability/drug effects
- Phage Therapy/veterinary*
- Rodent Diseases/drug therapy*
- Zebrafish
- PubMed
- 32453904 Full text @ Transbound. Emer. Dis.
Citation
Easwaran, M., De Zoysa, M., Shin, H.J. (2020) Application of phage therapy: Synergistic effect of phage EcSw (ΦEcSw) and antibiotic combination towards antibiotic-resistant Escherichia coli. Transboundary and Emerging diseases. 67(6):2809-2817.
Abstract
Bacteriophage therapy is acknowledged as a potential tool to prevent or treat multidrug resistant bacterial infections. In this study, our major focus was on the bacteriolytic activity of phage EcSw (ΦEcSw) against the emergence of the clinically important Escherichia coli Sw1 and E. coli O157:H7. The amount of the antibiotics were changed in a concentration dependent manner and the ΦEcSw susceptibility to antibiotics were determined. The kanamycin and chloramphenicol inhibited the titer of phage, but ampicillin did not show phage inhibition. Though the kanamycin and chloramphenicol controlled the growth of Sw1 in a concentration dependent manner, the ampicillin did not due to the resistance. The combined activity of the ΦEcSw with antibiotics (kanamycin and chloramphenicol) compared with the antibiotics alone showed significant lytic activity p <0.001). In addition, phage-based therapy was evaluated for controlling the multidrug resistant E. coli Sw1 and E. coli O157:H7 in zebrafish and BALB/c mice, respectively. Our results provide novel advantages of phage therapy and phage-antibiotic therapy to control antibiotic-resistant bacteria.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping