ZFIN ID: ZDB-PUB-200526-8
Developmental exposure to mepanipyrim induces locomotor hyperactivity in zebrafish (Danio rerio) larvae
Shen, C., Zhou, Y., Tang, C., He, C., Zuo, Z.
Date: 2020
Source: Chemosphere   256: 127106 (Journal)
Registered Authors: He, Chengyong
Keywords: Danio rerio, GABA, Locomotor hyperactivity, Mepanipyrim, Skeletal muscle contraction, Synaptic transmission
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian/drug effects
  • Fungicides, Industrial/toxicity*
  • Larva/drug effects
  • Locomotion/drug effects*
  • Pyrimidines/toxicity*
  • Zebrafish/metabolism
PubMed: 32447115 Full text @ Chemosphere
Mepanipyrim is a widely used fungicide, and residues of mepanipyrim are frequently detected in commodities. However, the neurotoxicity and underlying mechanisms of mepanipyrim are still insufficiently understood. In this study, zebrafish embryos at 0.5-1.0 post-fertilization hours (hpf) were exposed to 0.1, 1, 10 and 100 μg/L mepanipyrim for 7 days. Our results showed that mepanipyrim could cause the locomotor hyperactivity and increase the concentration of γ-amino butyric acid (GABA) and the Na+/K+- and Ca2+-ATPase activities in zebrafish larvae. We have conducted the RNA-sequence and RT-qPCR to analyze the gene expressions. The mRNA expression levels of calcium/sodium ion conduction associated genes were observably up-regulated, demonstrating that mepanipyrim could enhance the cell energy metabolism, the synaptic transmission and skeletal muscle contraction, which were consistent with the locomotor hyperactivity. Meanwhile, exposure to mepanipyrim could significantly change the gene expression levels of gad1, bdnf, nlgn1, and type A and B GABA receptors in zebrafish larvae. This is the first study focusing on the underlying mechanisms of the neurotoxic effects that are induced by mepanipyrim.