ZFIN ID: ZDB-PUB-200524-24
Effect of intracerebroventricular administration of two molecular forms of sulfated CCK octapeptide on anxiety-like behavior in the zebrafish danio rerio
Matsuda, K., Yoshida, D., Sachuriga, ., Watanabe, K., Yokobori, E., Konno, N., Nakamachi, T.
Date: 2020
Source: Peptides   130: 170330 (Journal)
Registered Authors: Matsuda, Kouhei, Yoshida, Tomoyuki
Keywords: Anxiety-Like behavior, Brain, CCK-like immunoreactivity, CCKA-8s, CCKB-8s, Cholecystokinin, ICV administration, Preference test, zebra fish
MeSH Terms:
  • Animals
  • Anxiety/chemically induced*
  • Behavior, Animal/drug effects*
  • Benzodiazepines/pharmacology
  • Brain/metabolism*
  • Carbolines/pharmacology
  • Injections, Intraventricular
  • Locomotion/drug effects
  • Proglumide/pharmacology
  • Sincalide/administration & dosage
  • Sincalide/analogs & derivatives*
  • Sincalide/metabolism
  • Sincalide/pharmacology
  • Zebrafish*
  • Zebrafish Proteins/metabolism
PubMed: 32445877 Full text @ Peptides
Cholecystokinin octapeptide with sulfate (CCK-8 s) regulates feeding behavior and psychomotor activity. In rodents and goldfish, intracerebroventricular (ICV) injection of CCK-8 s decreases food intake and also induces anxiety-like behavior. The zebrafish has several merits for investigating the psychophysiological roles of neuropeptides. However, little is known about the brain localization of CCK and the behavioral action of CCK-8 s in this species. Here we investigated the brain localization of CCK-like immunoreactivity and found that it was distributed throughout the brain. As CCK-like immunoreactivity was particularly evident in the ventral habenular nucleus, the interpeduncular nucleus and superior raphe, we subsequently examined the effect of zebrafish (zf) CCK-8 s on psychomotor control. Since the zebrafish possesses two molecular forms of zfCCK-8 s (zfCCKA-8 s and zfCCKB-8 s), two synthetic peptides were administered intracerebroventricularly at 1, 5 and 10 pmol g-1 body weight (BW). As the zebrafish shows a greater preference for the lower area of a tank than for to the upper area, we used this preference for assessment of anxiety-like behavior. ICV administration of zfCCKA-8 s or zfCCKB-8 s at 10 pmol g-1 BW significantly shortened the time spent in the upper area. The actions of these peptides mimicked that of the central-type benzodiazepine receptor inverse agonist FG-7142 (an anxiogenic agent) at 10 pmol g-1 BW. The anxiogenic-like action of the two peptides was attenuated by treatment with the CCK receptor antagonist proglumide at 200 pmol g-1 BW. These results indicate that zfCCKA-8 s and zfCCKB-8 s potently induce anxiety-like behavior via the CCK receptor-signaling pathway in the zebrafish brain.