Splicing- and demethylase-independent functions of LSD1 in zebrafish primitive hematopoiesis
- Tamaoki, J., Takeuchi, M., Abe, R., Kaneko, H., Wada, T., Hino, S., Nakao, M., Furukawa, Y., Kobayashi, M.
- Scientific Reports 10: 8521 (Journal)
- Registered Authors
- Kaneko, Hiroshi, Kobayashi, Makoto, Takeuchi, Miki, Tamaoki, Junya
- MeSH Terms
- Amino Acid Sequence
- Cells, Cultured
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology*
- Histone Demethylases/genetics
- Histone Demethylases/metabolism*
- Protein Isoforms
- RNA Splicing*
- Sequence Homology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- 32444613 Full text @ Sci. Rep.
Tamaoki, J., Takeuchi, M., Abe, R., Kaneko, H., Wada, T., Hino, S., Nakao, M., Furukawa, Y., Kobayashi, M. (2020) Splicing- and demethylase-independent functions of LSD1 in zebrafish primitive hematopoiesis. Scientific Reports. 10:8521.
LSD1/KDM1A is a widely conserved lysine-specific demethylase that removes methyl groups from methylated proteins, mainly histone H3. We previously isolated the zebrafish LSD1 gene and demonstrated that it is required for primitive hematopoiesis. Recently, a neuron-specific splicing variant of LSD1 was found in mammals and its specific functions and substrate specificities were reported. To our surprise, zebrafish LSD1 cDNA, which we previously analyzed, was corresponded to the neuron-specific variant in mammals. In this study, we investigated the structures and expression of LSD1 splicing variants in zebrafish and found all 4 types of LSD1 isoforms: LSD1, LSD1+2al, LSD1+8al and LSD1+2al8al. Interestingly, LSD1+8al/LSD1+2al8al, which correspond to mammalian neuron-specific variants, expressed ubiquitously in zebrafish. We also performed phenotypic rescue experiments of a zebrafish LSD1 mutant (kdm1ait627) using human and zebrafish LSD1 variants to identify which variant is involved in primitive hematopoiesis. Unexpectedly, the overexpression of all types of human and zebrafish variants was able to rescue the hematopoietic phenotypes in LSD1 mutants. Furthermore, enzymatic-deficient LSD1K661A (human) and K638A (zebrafish) were also able to rescue the mutant phenotypes. These results suggest that the LSD1 functions in zebrafish primitive hematopoiesis are free from any splicing-dependent regulation or demethylation reaction.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes