PUBLICATION

Shootin-1 Is Required for Nervous System Development in Zebrafish

Authors
Emerson, S.E., Stergas, H.R., Bupp-Chickering, S.O., Ebert, A.M.
ID
ZDB-PUB-200515-6
Date
2020
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   249(10): 1285-1295 (Journal)
Registered Authors
Keywords
PlexinA2, Retina, Semaphorin6A, cytoskeleton, microarray, migration
MeSH Terms
  • Peripheral Nervous System/physiology
  • Humans
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology*
  • Cell Movement
  • Nervous System/embryology*
  • Zebrafish
  • Retinal Pigment Epithelium/physiology
  • Neurons/metabolism
  • Motor Neurons/metabolism
  • Axons/physiology
  • Body Patterning
  • Semaphorins/genetics
  • Semaphorins/physiology*
  • Animals
  • Cytoskeletal Proteins/genetics
  • Cytoskeletal Proteins/physiology*
  • Phenotype
  • Gene Expression Regulation, Developmental*
(all 21)
PubMed
32406957 Full text @ Dev. Dyn.
Abstract
Background Semaphorin6A (Sema6A) and its PlexinA2 (PlxnA2) receptor canonically function as repulsive axon guidance cues. To understand downstream signaling mechanisms, we performed a microarray screen and identified the "clutch molecule" shootin-1 (shtn-1) as a transcriptionally repressed target (Emerson et al., 2017). Shtn-1 is a key proponent of cell migration and neuronal polarization and must be regulated during nervous system development (Toriyama et al., 2006; Shimada et al., 2008; Kubo et al., 2015) The mechanisms of Shtn-1 regulation and the phenotypic consequences of loss of repression are poorly understood.
Results We demonstrate shtn-1 overexpression results in impaired migration of the optic vesicles, lack of retinal pigmented epithelium, and pathfinding errors of retinotectal projections. We also observed patterning defects in the peripheral nervous system. Importantly, these phenotypes were rescued by overexpressing PlxnA2.
Conclusions We demonstrate a functional role for repression of shtn-1 by PlxnA2 in development of the eyes and peripheral nervous system in zebrafish. These results demonstrate that careful regulation of shtn-1 is critical for development of the nervous system. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
GFPsb2Tgstandard conditionsProtruding-mouthIFL
GFPzf460Tgstandard conditions14-19 somitesIFL
GFPzf460Tgstandard conditions14-19 somitesIFL
GFPzf460Tg + MO1-plxna2standard conditions14-19 somitesIFL
mCherryzf3031Tgstandard conditionsProtruding-mouthIFL
plxna2WTstandard conditions14-19 somitesISH
plxna2WTstandard conditionsProtruding-mouthISH
plxna2WTstandard conditionsProtruding-mouthISH
RFPcu2Tgstandard conditionsProtruding-mouthIFL
RFPcu2Tgstandard conditionsProtruding-mouthIFL
1 - 10 of 20
Show
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-plxna2 + MO4-tp53standard conditions14-19 somites
zf460Tg + MO1-plxna2standard conditions14-19 somites
1 - 2 of 2
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cu2TgTransgenic Insertion
    sb2TgTransgenic Insertion
      zf460TgTransgenic Insertion
        zf3031TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          plxna2MO1-plxna2MRPHLNO
          tp53MO4-tp53MRPHLNO
          1 - 2 of 2
          Show
          Fish
          Fish
          cu2Tg
          sb2Tg
          WT
          WT + MO1-plxna2 + MO4-tp53
          zf460Tg
          zf460Tg + MO1-plxna2
          zf3031Tg
          1 - 7 of 7
          Show
          Antibodies
          No data available
          Orthology
          Gene Orthology
          plxna2
          1 - 1 of 1
          Show
          Engineered Foreign Genes
          Marker Marker Type Name
          GFPEFGGFP
          mCherryEFGmCherry
          RFPEFGRFP
          1 - 3 of 3
          Show
          Mapping
          No data available
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          Citation
          Emerson, S.E., Stergas, H.R., Bupp-Chickering, S.O., Ebert, A.M. (2020) Shootin-1 Is Required for Nervous System Development in Zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 249(10):1285-1295.