PUBLICATION

CRISPR mutation in lysine (K)-specific demethylase 1a (kdm1a/LSD1) does not produce defects to skeletal development in maternal zygotic mutant zebrafish larvae

Authors
DeLaurier A., Wiggins K.J., Mishoe Hernandez L., Loyo Rosado F., Pugh, K., Shabdue, C.
ID
ZDB-PUB-200515-15
Date
2020
Source
ZebraShare : (Unpublished)
Registered Authors
DeLaurier, April
Keywords
none
MeSH Terms
none
PubMed
none
Abstract
CRISPR-Cas9 technology was used to generate a mutation in exon 1 of kdm1a (LSD1). A 14 bp deletion was generated, causing missense and a premature stop codon predicted to cause a truncated protein. Mutants are viable and able to reproduce. Maternal zygotic mutants were generated and examined for skeletal phenotypes using Alician Blue and Alizarin Red dyes. No obvious skeletal defects were observed in fish at 4 days post-fertilization.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
kdm1aaik5/aik5 (AB)standard conditionsDay 4
kdm1aaik5/aik5 (AB)standard conditionsDay 4
kdm1aaik5/aik5 (AB)standard conditionsDay 4
1 - 3 of 3
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
aik5
    		Small Deletion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    kdm1aCRISPR1-kdm1aCRISPR
    1 - 1 of 1
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    Fish
    Fish
    kdm1aaik5/aik5 (AB)
    1 - 1 of 1
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    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    DeLaurier A., Wiggins K.J., Mishoe Hernandez L., Loyo Rosado F., Pugh, K., Shabdue, C. (2020) CRISPR mutation in lysine (K)-specific demethylase 1a (kdm1a/LSD1) does not produce defects to skeletal development in maternal zygotic mutant zebrafish larvae. ZebraShare. .