PUBLICATION
The Influence of Behavioral, Social, and Environmental Factors on Reproducibility and Replicability in Aquatic Animal Models
- Authors
- Lieggi, C., Kalueff, A.V., Lawrence, C., Collymore, C.
- ID
- ZDB-PUB-200514-6
- Date
- 2020
- Source
- ILAR journal 60(2): 270-288 (Review)
- Registered Authors
- Kalueff, Allan V., Lawrence, Christian
- Keywords
- behavior, enrichment, husbandry, neurobehavior, replicability, reproducibility, xenopus; zebrafish
- MeSH Terms
-
- Animal Welfare/statistics & numerical data
- Animals
- Behavior, Animal/physiology
- Housing, Animal/statistics & numerical data
- Models, Animal
- Reproducibility of Results
- Xenopus/physiology
- Zebrafish/physiology*
- PubMed
- 32400880 Full text @ ILAR (Institute for Laboratory Animal Research)
Citation
Lieggi, C., Kalueff, A.V., Lawrence, C., Collymore, C. (2020) The Influence of Behavioral, Social, and Environmental Factors on Reproducibility and Replicability in Aquatic Animal Models. ILAR journal. 60(2):270-288.
Abstract
The publication of reproducible, replicable, and translatable data in studies utilizing animal models is a scientific, practical, and ethical necessity. This requires careful planning and execution of experiments and accurate reporting of results. Recognition that numerous developmental, environmental, and test-related factors can affect experimental outcomes is essential for a quality study design. Factors commonly considered when designing studies utilizing aquatic animal species include strain, sex, or age of the animal; water quality; temperature; and acoustic and light conditions. However, in the aquatic environment, it is equally important to consider normal species behavior, group dynamics, stocking density, and environmental complexity, including tank design and structural enrichment. Here, we will outline normal species and social behavior of 2 commonly used aquatic species: zebrafish (Danio rerio) and Xenopus (X. laevis and X. tropicalis). We also provide examples as to how these behaviors and the complexity of the tank environment can influence research results and provide general recommendations to assist with improvement of reproducibility and replicability, particularly as it pertains to behavior and environmental complexity, when utilizing these popular aquatic models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping