PUBLICATION
Transient and lineage-restricted requirement of Ebf3 for sternum ossification
- Authors
- Kuriki, M., Sato, F., Arai, H.N., Sogabe, M., Kaneko, M., Kiyonari, H., Kawakami, K., Yoshimoto, Y., Shukunami, C., Sehara-Fujisawa, A.
- ID
- ZDB-PUB-200514-3
- Date
- 2020
- Source
- Development (Cambridge, England) 147(9): (Journal)
- Registered Authors
- Kaneko, Maki, Kawakami, Koichi, Sehara-Fujisawa, Atsuko, Shukunami, Chisa
- Keywords
- Fibroblast, Lateral plate mesenchyme cells, Pre-osteoblast, Runx2, Scleraxis, Sternum
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Core Binding Factor Alpha 1 Subunit/metabolism
- Embryo, Nonmammalian/metabolism
- Female
- Fibroblasts/metabolism
- In Situ Hybridization
- LIM-Homeodomain Proteins/metabolism
- Mice
- Mice, Knockout
- Osteoblasts/metabolism
- Pregnancy
- RNA-Seq
- Sternum/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 32398354 Full text @ Development
Citation
Kuriki, M., Sato, F., Arai, H.N., Sogabe, M., Kaneko, M., Kiyonari, H., Kawakami, K., Yoshimoto, Y., Shukunami, C., Sehara-Fujisawa, A. (2020) Transient and lineage-restricted requirement of Ebf3 for sternum ossification. Development (Cambridge, England). 147(9):.
Abstract
Osteoblasts arise from bone-surrounding connective tissue containing tenocytes and fibroblasts. Lineages of these cell populations and mechanisms of their differentiation are not well understood. Screening enhancer-trap lines of zebrafish allowed us to identify Ebf3 as a transcription factor marking tenocytes and connective tissue cells in skeletal muscle of embryos. Knockout of Ebf3 in mice had no effect on chondrogenesis but led to sternum ossification defects as a result of defective generation of Runx2+ pre-osteoblasts. Conditional and temporal Ebf3 knockout mice revealed requirements of Ebf3 in the lateral plate mesenchyme cells (LPMs), especially in tendon/muscle connective tissue cells, and a stage-specific Ebf3 requirement at embryonic day 9.5-10.5. Upregulated expression of connective tissue markers, such as Egr1/2 and Osr1, increased number of Islet1+ mesenchyme cells, and downregulation of gene expression of the Runx2 regulator Shox2 in Ebf3-deleted thoracic LPMs suggest crucial roles of Ebf3 in the onset of lateral plate mesoderm differentiation towards osteoblasts forming sternum tissues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping