PUBLICATION

Transient and lineage-restricted requirement of Ebf3 for sternum ossification

Authors
Kuriki, M., Sato, F., Arai, H.N., Sogabe, M., Kaneko, M., Kiyonari, H., Kawakami, K., Yoshimoto, Y., Shukunami, C., Sehara-Fujisawa, A.
ID
ZDB-PUB-200514-3
Date
2020
Source
Development (Cambridge, England)   147(9): (Journal)
Registered Authors
Kaneko, Maki, Kawakami, Koichi, Sehara-Fujisawa, Atsuko, Shukunami, Chisa
Keywords
Fibroblast, Lateral plate mesenchyme cells, Pre-osteoblast, Runx2, Scleraxis, Sternum
MeSH Terms
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Core Binding Factor Alpha 1 Subunit/metabolism
  • Embryo, Nonmammalian/metabolism
  • Female
  • Fibroblasts/metabolism
  • In Situ Hybridization
  • LIM-Homeodomain Proteins/metabolism
  • Mice
  • Mice, Knockout
  • Osteoblasts/metabolism
  • Pregnancy
  • RNA-Seq
  • Sternum/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
32398354 Full text @ Development
Abstract
Osteoblasts arise from bone-surrounding connective tissue containing tenocytes and fibroblasts. Lineages of these cell populations and mechanisms of their differentiation are not well understood. Screening enhancer-trap lines of zebrafish allowed us to identify Ebf3 as a transcription factor marking tenocytes and connective tissue cells in skeletal muscle of embryos. Knockout of Ebf3 in mice had no effect on chondrogenesis but led to sternum ossification defects as a result of defective generation of Runx2+ pre-osteoblasts. Conditional and temporal Ebf3 knockout mice revealed requirements of Ebf3 in the lateral plate mesenchyme cells (LPMs), especially in tendon/muscle connective tissue cells, and a stage-specific Ebf3 requirement at embryonic day 9.5-10.5. Upregulated expression of connective tissue markers, such as Egr1/2 and Osr1, increased number of Islet1+ mesenchyme cells, and downregulation of gene expression of the Runx2 regulator Shox2 in Ebf3-deleted thoracic LPMs suggest crucial roles of Ebf3 in the onset of lateral plate mesoderm differentiation towards osteoblasts forming sternum tissues.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes