ZFIN ID: ZDB-PUB-200506-5
RNA-seq analysis and compound screening highlight multiple signalling pathways regulating secondary cell death after acute CNS injury in vivo
Herzog, C., Greenald, D., Larraz, J., Keatinge, M., Herrgen, L.
Date: 2020
Source: Biology Open   9(5): (Journal)
Registered Authors: Herrgen, Leah, Keatinge, Marcus
Keywords: CNS injury, Cellular signalling, Compound screen, RNA-seq, Secondary cell death, Zebrafish
Microarrays: GEO:GSE140810
MeSH Terms:
  • Animals
  • Biomarkers
  • Brain Injuries/etiology*
  • Brain Injuries/metabolism*
  • Brain Injuries/pathology
  • Cell Death/genetics*
  • Cytokines/genetics
  • Cytokines/metabolism
  • Disease Susceptibility*
  • Gene Expression Regulation
  • Gene Knockdown Techniques
  • Larva
  • Macrophages/metabolism
  • Neurons/metabolism
  • RNA-Seq
  • Signal Transduction
  • Spinal Cord Injuries/etiology*
  • Spinal Cord Injuries/metabolism*
  • Spinal Cord Injuries/pathology
  • Transcriptome
  • Zebrafish
PubMed: 32366533 Full text @ Biol. Open
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ABSTRACT
Understanding the molecular mechanisms that regulate secondary cell death after acute central nervous system (CNS) injury is critical for the development of effective neuroprotective drugs. Previous research has shown that neurotoxic processes including excitotoxicity, oxidative stress and neuroinflammation can cause secondary cell death. Nevertheless, clinical trials targeting these processes have been largely unsuccessful, suggesting that the signalling pathways underlying secondary cell death remain incompletely understood. Due to their suitability for live imaging and their amenability to genetic and pharmacological manipulation, larval zebrafish provide an ideal platform for studying the regulation of secondary cell death in vivo Here, we use RNA-seq gene expression profiling and compound screening to identify signalling pathways that regulate secondary cell death after acute neural injury in larval zebrafish. RNA-seq analysis of genes upregulated in cephalic mpeg1+ macrophage-lineage cells isolated from mpeg1:GFP transgenic larvae after neural injury suggested an involvement of cytokine and polyamine signalling in secondary cell death. Furthermore, screening a library of FDA approved compounds indicated roles for GABA, serotonin and dopamine signalling. Overall, our results highlight multiple signalling pathways that regulate secondary cell death in vivo, and thus provide a starting point for the development of novel neuroprotective treatments for patients with CNS injury.This article has an associated First Person interview with the two first authors of the paper.
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