PUBLICATION
Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish
- Authors
- Makarova, N., Kalaparthi, V., Wang, A., Williams, C., Dokukin, M.E., Kaufman, C.K., Zon, L., Sokolov, I.
- ID
- ZDB-PUB-200505-6
- Date
- 2020
- Source
- Journal of the Mechanical Behavior of Biomedical Materials 107: 103746 (Journal)
- Registered Authors
- Zon, Leonard I.
- Keywords
- Atomic force microscopy, Cell mechanics, Initiation of cancer, Pericellular coat, Zebrafish
- MeSH Terms
-
- Animals
- Cell Transformation, Neoplastic
- Elastic Modulus
- Elasticity
- Melanoma*/genetics
- Microscopy, Atomic Force
- Zebrafish*/genetics
- PubMed
- 32364948 Full text @ J. Mech. Behav. Biomed. Mater.
Citation
Makarova, N., Kalaparthi, V., Wang, A., Williams, C., Dokukin, M.E., Kaufman, C.K., Zon, L., Sokolov, I. (2020) Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish. Journal of the Mechanical Behavior of Biomedical Materials. 107:103746.
Abstract
Despite sharing oncogenetic mutations, only a small number of cells within a given tissue will undergo malignant transformation. Biochemical and physical factors responsible for this cancer-initiation process are not well understood. Here we study biophysical differences of pre-melanoma and melanoma cells in a BRAFV600E/P53 zebrafish model. The AFM indentation technique was used to study the cancer-initiating cells while the surrounding melanocytes were the control. We observed a statistically significant decrease in the modulus of elasticity (the effective Young's modulus) of cancer-initiating cells compared to the surrounding melanocytes. No significant differences in the pericellular coat surrounding cells were observed. These results contribute to a better understanding of the factors responsible for the initiation of cancer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping