ZFIN ID: ZDB-PUB-200505-6
Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish
Makarova, N., Kalaparthi, V., Wang, A., Williams, C., Dokukin, M.E., Kaufman, C.K., Zon, L., Sokolov, I.
Date: 2020
Source: Journal of the Mechanical Behavior of Biomedical Materials   107: 103746 (Journal)
Registered Authors: Zon, Leonard I.
Keywords: Atomic force microscopy, Cell mechanics, Initiation of cancer, Pericellular coat, Zebrafish
MeSH Terms:
  • Animals
  • Cell Transformation, Neoplastic
  • Elastic Modulus
  • Elasticity
  • Melanoma*/genetics
  • Microscopy, Atomic Force
  • Zebrafish*/genetics
PubMed: 32364948 Full text @ J. Mech. Behav. Biomed. Mater.
Despite sharing oncogenetic mutations, only a small number of cells within a given tissue will undergo malignant transformation. Biochemical and physical factors responsible for this cancer-initiation process are not well understood. Here we study biophysical differences of pre-melanoma and melanoma cells in a BRAFV600E/P53 zebrafish model. The AFM indentation technique was used to study the cancer-initiating cells while the surrounding melanocytes were the control. We observed a statistically significant decrease in the modulus of elasticity (the effective Young's modulus) of cancer-initiating cells compared to the surrounding melanocytes. No significant differences in the pericellular coat surrounding cells were observed. These results contribute to a better understanding of the factors responsible for the initiation of cancer.