PUBLICATION
A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos
- Authors
- Somkhit, J., Loyant, R., Brenet, A., Hassan-Abdi, R., Yanicostas, C., Porceddu, M., Borgne-Sanchez, A., Soussi-Yanicostas, N.
- ID
- ZDB-PUB-200505-5
- Date
- 2020
- Source
- Zebrafish 17(4): 268-270 (Journal)
- Registered Authors
- Soussi-Yanicostas, Nadia
- Keywords
- oxygen consumption, respiration, zebrafish larvae
- MeSH Terms
-
- Animals
- Antimycin A/pharmacology*
- Embryo, Nonmammalian/metabolism*
- Larva/metabolism
- Oxygen Consumption*
- Rotenone/pharmacology*
- Zebrafish/embryology
- Zebrafish/growth & development
- Zebrafish/metabolism*
- PubMed
- 32364833 Full text @ Zebrafish
Citation
Somkhit, J., Loyant, R., Brenet, A., Hassan-Abdi, R., Yanicostas, C., Porceddu, M., Borgne-Sanchez, A., Soussi-Yanicostas, N. (2020) A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos. Zebrafish. 17(4):268-270.
Abstract
In all animal species, oxygen consumption is a key process that is partially impaired in a large number of pathological situations and thus provides informative details on the physiopathology of the disease. In this study, we describe a simple and affordable method to precisely measure oxygen consumption in living zebrafish larvae using a spectrofluorometer and the MitoXpress Xtra Oxygen Consumption Assay. In addition, we used zebrafish larvae treated with mitochondrial respiratory chain inhibitors, antimycin A or rotenone, to verify that our method enables precise and reliable measurements of oxygen consumption.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping