PUBLICATION

Deep three-photon imaging of the brain in intact adult zebrafish

Authors
Chow, D.M., Sinefeld, D., Kolkman, K.E., Ouzounov, D.G., Akbari, N., Tatarsky, R., Bass, A., Xu, C., Fetcho, J.R.
ID
ZDB-PUB-200429-5
Date
2020
Source
Nature Methods   17(6): 605-608 (Journal)
Registered Authors
Fetcho, Joseph R.
Keywords
none
MeSH Terms
  • Animals
  • Cerebellum/diagnostic imaging*
  • Imaging, Three-Dimensional/methods*
  • Photons*
  • Superior Colliculi/diagnostic imaging*
  • Telencephalon/diagnostic imaging*
  • Zebrafish/anatomy & histology*
PubMed
32341543 Full text @ Nat. Methods
Abstract
Behaviors emerge from activity throughout the brain, but noninvasive optical access in adult vertebrate brains is limited. We show that three-photon (3P) imaging through the head of intact adult zebrafish allows structural and functional imaging at cellular resolution throughout the telencephalon and deep into the cerebellum and optic tectum. With 3P imaging, considerable portions of the brain become noninvasively accessible from embryo to sexually mature adult in a vertebrate model.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes