PUBLICATION
Network pharmacology approach to elucidate possible action mechanisms of Sinomenii Caulis for treating osteoporosis
- Authors
- Liu, W.J., Jiang, Z.M., Chen, Y., Xiao, P.T., Wang, Z.Y., Huang, T.Q., Liu, E.H.
- ID
- ZDB-PUB-200424-9
- Date
- 2020
- Source
- Journal of ethnopharmacology 257: 112871 (Journal)
- Registered Authors
- Keywords
- Network pharmacology, Osteoporosis, Sinomenii caulis
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/pharmacology
- Bone Density Conservation Agents/pharmacology*
- Bone Remodeling/drug effects*
- Bone Remodeling/genetics
- Bone and Bones/drug effects*
- Bone and Bones/metabolism
- Bone and Bones/physiopathology
- Cytokines/genetics
- Cytokines/metabolism
- Databases, Protein
- Disease Models, Animal
- Drugs, Chinese Herbal/pharmacology*
- Gene Expression Regulation
- Gene Regulatory Networks
- Inflammation Mediators/metabolism
- Osteoporosis/chemically induced
- Osteoporosis/genetics
- Osteoporosis/metabolism
- Osteoporosis/physiopathology
- Osteoprotegerin/genetics
- Osteoprotegerin/metabolism
- Prednisolone
- Protein Interaction Maps
- RANK Ligand/genetics
- RANK Ligand/metabolism
- Receptor Activator of Nuclear Factor-kappa B/genetics
- Receptor Activator of Nuclear Factor-kappa B/metabolism
- Signal Transduction
- Systems Biology*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32325182 Full text @ J. Ethnopharmacol.
Citation
Liu, W.J., Jiang, Z.M., Chen, Y., Xiao, P.T., Wang, Z.Y., Huang, T.Q., Liu, E.H. (2020) Network pharmacology approach to elucidate possible action mechanisms of Sinomenii Caulis for treating osteoporosis. Journal of ethnopharmacology. 257:112871.
Abstract
Ethnopharmacological relevance Sinomenii Caulis (SC) is a well-konwn traditional Chinese medicine used for treatment of rheumatoid arthritis (RA), dermatophytosis and paralysis. Patients with RA are usually secondary to osteoporosis, but the potential protective effect of SC on osteoporosis (OP) is seldom reported and its possible action mechanism is little known.
Aim The purpose of this study was to demonstrate the anti-osteoporosis effects of SC extract and alkaloids in prednisolone (Pre)-induced OP of zebrafish, and then to explore the potential mechanism of SC on system level by network pharmacology.
Methods Firstly, zebrafish OP model was established to investigate the anti-osteoporosis effect of SC. Secondly, the targets of SC and OP from multiple databases were collected, and Compound-Target-Pathway network based on protein-protein interaction (PPI) was constructed. Moreover, Gene enrichment and annotation were performed via the DAVID server. Finally, the reliability of the network pharmacology prediction results in Pre-induced OP of zebrafish was verified by qRT-PCR.
Results The results indicated that SC extract and alkaloids have remarkable ability to promote bone formation of cranial bones and reduce TRAP contents in Pre-induced OP of zebrafish. 32 OP-related ingredients in SC and 77 OP-related targets were screened from multiple databases, and 15 OP-related pathways were enriched by the KEGG database. Further experimental validation indicated that SC extract and alkaloids could regulate the expression of MAPK14, CASP3, CXCL8, IL-1β, IL6, PTGS2, TNF-α, ESR1, and MMP9 for treatment of OP.
Conclusion In summary, we conducted an integrative analysis to provide convincing evidence that SC may partially alleviate OP by inhibiting pro-inflammatory cytokines and regulating of RANK/RANKL/OPG system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping