PUBLICATION

Neuropeptide Y deficiency induces anxiety-like behaviours in zebrafish (Danio rerio)

Authors
Shiozaki, K., Kawabe, M., Karasuyama, K., Kurachi, T., Hayashi, A., Ataka, K., Iwai, H., Takeno, H., Hayasaka, O., Kotani, T., Komatsu, M., Inui, A.
ID
ZDB-PUB-200422-23
Date
2020
Source
Scientific Reports   10: 5913 (Journal)
Registered Authors
Shiozaki, Kazuhiro
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Anxiety/genetics*
  • Anxiety/physiopathology
  • Anxiety/psychology
  • Behavior, Animal/physiology
  • Catecholamines/metabolism
  • Disease Models, Animal
  • Energy Metabolism/physiology*
  • Female
  • Gene Knockout Techniques
  • Humans
  • Male
  • Neuropeptide Y/deficiency*
  • Neuropeptide Y/genetics
  • Orexins/metabolism
  • Receptors, Neuropeptide Y/metabolism
  • Signal Transduction/physiology
  • Stress, Psychological/physiopathology*
  • Zebrafish
  • Zebrafish Proteins/metabolism
PubMed
32246073 Full text @ Sci. Rep.
Abstract
Neuropeptide Y (NPY) controls energy homeostasis including orexigenic actions in mammalians and non-mammalians. Recently, NPY has attracted attention as a mediator of emotional behaviour and psychosomatic diseases. However, its functions are not fully understood. We established npy gene-deficient (NPY-KO) zebrafish (Danio rerio) to assess the relationship between NPY and emotional behaviours. The NPY-KO zebrafish exhibited similar growth, but pomc and avp mRNA levels in the brain were higher as compared to wild-type fish. NPY-KO zebrafish exhibited several anxiety-like behaviours, such as a decrease in social interaction in mirror test and decreased locomotion in black-white test. The acute cold stress-treated NPY-KO zebrafish exhibited anxiety-like behaviours such as remaining stationary and swimming along the side of the tank in the mirror test. Moreover, expression levels of anxiety-associated genes (orx and cck) and catecholamine production (gr, mr, th1 and th2) were significantly higher in NPY-KO zebrafish than in wild-type fish. We demonstrated that NPY-KO zebrafish have an anxiety phenotype and a stress-vulnerability like NPY-KO mice, whereby orx and/or catecholamine signalling may be involved in the mechanism actions.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping