ZFIN ID: ZDB-PUB-200403-95
Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish
Singh, S.P., Ninov, N.
Date: 2020
Source: Methods in molecular biology (Clifton, N.J.)   2128: 159-179 (Review)
Registered Authors: Ninov, Nikolay, Singh, Sumeet Pal
Keywords: Brainbow, Heterogeneity, Lineage tracing, Quiescence, Single cell
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Lineage
  • Cell Proliferation*
  • Cell Tracking/methods*
  • Cloning, Molecular/methods
  • Color
  • Genes, Reporter
  • Image Processing, Computer-Assisted/methods*
  • Insulin-Secreting Cells/chemistry
  • Insulin-Secreting Cells/cytology*
  • Integrases
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Microscopy, Confocal/methods*
  • Models, Animal
  • Staining and Labeling/methods*
  • Zebrafish
PubMed: 32180193 Full text @ Meth. Mol. Biol.
ABSTRACT
During embryogenesis, beta-cells arise from the dorsal and ventral bud originating in the endoderm germ layer. As the animal develops to adulthood, the beta-cell mass dramatically increases. The expansion of the beta-cell population is driven by cell division among the embryonic beta-cells and supplanted by neogenesis from post-embryonic progenitors. Here, we describe a protocol for multicolor clonal analysis in zebrafish to define the contribution of individual embryonic beta-cells to the increase in cell numbers. This technique provides insights into the proliferative history of individual beta-cells in an islet. This insight helps in defining the replicative heterogeneity among individual beta-cells during development. Additionally, the ability to discriminate individual cells based on unique color signatures helps quantify the volume occupied by beta-cells and define the contribution of cellular size to the beta-cell mass.
ADDITIONAL INFORMATION No data available