ZFIN ID: ZDB-PUB-200403-63
BEL β-Trefoil Reduces the Migration Ability of RUNX2 Expressing Melanoma Cells in Xenotransplanted Zebrafish
Valenti, M.T., Marchetto, G., Perduca, M., Tiso, N., Mottes, M., Dalle Carbonare, L.
Date: 2020
Source: Molecules   25(6): (Journal)
Registered Authors: Tiso, Natascia
Keywords: BEL β-trefoil, RUNX2, lectin, melanoma, zebrafish
MeSH Terms:
  • Animals
  • Antineoplastic Agents/metabolism
  • Antineoplastic Agents/pharmacology*
  • Basidiomycota/chemistry
  • Cell Line, Tumor
  • Cell Movement/drug effects
  • Cell Proliferation/drug effects
  • Cloning, Molecular
  • Core Binding Factor Alpha 1 Subunit/genetics*
  • Core Binding Factor Alpha 1 Subunit/metabolism
  • Embryo, Nonmammalian
  • Escherichia coli/genetics
  • Escherichia coli/metabolism
  • Fungal Proteins/genetics
  • Fungal Proteins/metabolism
  • Fungal Proteins/pharmacology*
  • Gene Expression
  • Genetic Vectors/chemistry
  • Genetic Vectors/metabolism
  • Humans
  • Melanocytes/drug effects
  • Melanocytes/metabolism
  • Melanocytes/pathology
  • Melanoma/drug therapy*
  • Melanoma/genetics
  • Melanoma/metabolism
  • Melanoma/pathology
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Recombinant Fusion Proteins/pharmacology
  • Skin Neoplasms/drug therapy*
  • Skin Neoplasms/genetics
  • Skin Neoplasms/metabolism
  • Skin Neoplasms/pathology
  • Trefoil Factors/genetics
  • Trefoil Factors/metabolism
  • Trefoil Factors/pharmacology*
  • Xenograft Model Antitumor Assays
  • Zebrafish
PubMed: 32168858 Full text @ Molecules
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ABSTRACT
RUNX2, a master osteogenic transcript ion factor, is overexpressed in several cancer cells; in melanoma it promotes cells migration and invasion as well as neoangiogenesis. The annual mortality rates related to metastatic melanoma are high and novel agents are needed to improve melanoma patients' survival. It has been shown that lectins specifically target malignant cells since they present the Thomsen-Friedenreich antigen. This disaccharide is hidden in normal cells, while it allows selective lectins binding in transformed cells. Recently, an edible lectin named BEL β-trefoil has been obtained from the wild mushroom Boletus edulis. Our previous study showed BEL β-trefoil effects on transcription factor RUNX2 downregulation as well as on the migration ability in melanoma cells treated in vitro. Therefore, to better understand the role of this lectin, we investigated the BEL β-trefoil effects in a zebrafish in vivo model, transplanted with human melanoma cells expressing RUNX2. Our data showed that BEL β-trefoil is able to spread in the tissues and to reduce the formation of metastases in melanoma xenotransplanted zebrafish. In conclusion, BEL β-trefoil can be considered an effective biomolecule to counteract melanoma disease.
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