PUBLICATION
Environmental contaminants modulate transport activity of zebrafish organic anion transporters Oat1 and Oat3
- Authors
- Dragojević, J., Marić, P., Lončar, J., Popović, M., Mihaljević, I., Smital, T.
- ID
- ZDB-PUB-200403-53
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 231: 108742 (Journal)
- Registered Authors
- Smital, Tvrtko
- Keywords
- Cytotoxicity, Environmental contaminants, Interaction screening, Oat1 and Oat3 transporters, Stable transfectants, Zebrafish
- MeSH Terms
-
- Animals
- Biological Transport
- Environmental Pollutants/metabolism*
- HEK293 Cells
- Humans
- Organic Anion Transport Protein 1/metabolism*
- Organic Anion Transporters, Sodium-Independent/metabolism*
- Protein Binding
- Zebrafish/metabolism*
- PubMed
- 32165351 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Dragojević, J., Marić, P., Lončar, J., Popović, M., Mihaljević, I., Smital, T. (2020) Environmental contaminants modulate transport activity of zebrafish organic anion transporters Oat1 and Oat3. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 231:108742.
Abstract
Organic anion transporters (OATs) are transmembrane proteins which belong to SLC22 subfamily. They are responsible for the uptake of various endo- and xenobiotics into the cells of different organs and tissues. Following our previous work on characterization of zebrafish Oat1 and Oat3, in this study we analyzed interaction of various classes of environmental contaminants with these membrane transporters using the transport activity assay with HEK293 Flp-In cell line stably overexpressing zebrafish Oat1 and Oat3, respectively. Based on the initial screening of a series of 36 environmental contaminants on their ability to interact with zebrafish Oat1 and Oat3, the most potent interactors were selected, their IC50 values calculated and type of interaction determined. Finally, to further confirm the type of interaction and initially evaluate their toxic potential, the cytotoxicity assays were performed. Broad ligand selectivity and similarity of zebrafish Oat1 and Oat3 with mammalian orthologs was confirmed and potent interactors among environmental contaminants identified.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping